Journal article
Fascin Regulates Nuclear Movement and Deformation in Migrating Cells
Developmental cell, Vol.38(4), pp.371-383
08/22/2016
DOI: 10.1016/j.devcel.2016.07.021
PMCID: PMC4997957
PMID: 27554857
Abstract
Fascin is an F-actin-bundling protein shown to stabilize filopodia and regulate adhesion dynamics in migrating cells, and its expression is correlated with poor prognosis and increased metastatic potential in a number of cancers. Here, we identified the nuclear envelope protein nesprin-2 as a binding partner for fascin in a range of cell types in vitro and in vivo. Nesprin-2 interacts with fascin through a direct, F-actin-independent interaction, and this binding is distinct and separable from a role for fascin within filopodia at the cell periphery. Moreover, disrupting the interaction between fascin and nesprin-2 C-terminal domain leads to specific defects in F-actin coupling to the nuclear envelope, nuclear movement, and the ability of cells to deform their nucleus to invade through confined spaces. Together, our results uncover a role for fascin that operates independently of filopodia assembly to promote efficient cell migration and invasion.
Details
- Title: Subtitle
- Fascin Regulates Nuclear Movement and Deformation in Migrating Cells
- Creators
- Asier Jayo - Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guys Campus, London SE1 1UL, UKMajid Malboubi - London Centre for Nanotechnology, University College London, London WC1H 0AH, UKSusumu Antoku - Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USAWakam Chang - Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USAElena Ortiz-Zapater - Division of Asthma, Allergy & Lung Biology, King's College London, 5th Floor Tower Wing, Guy's Hospital Campus, London SE1 1UL, UKChristopher Groen - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAKarin Pfisterer - Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guys Campus, London SE1 1UL, UKTina Tootle - Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAGuillaume Charras - London Centre for Nanotechnology, University College London, London WC1H 0AH, UK; Department of Cell and Developmental Biology, University College London, London WC1E 6BT, UKGregg G Gundersen - Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USAMaddy Parsons - Randall Division of Cell and Molecular Biophysics, King's College London, New Hunt's House, Guys Campus, London SE1 1UL, UK. Electronic address: maddy.parsons@kcl.ac.uk
- Resource Type
- Journal article
- Publication Details
- Developmental cell, Vol.38(4), pp.371-383
- DOI
- 10.1016/j.devcel.2016.07.021
- PMID
- 27554857
- PMCID
- PMC4997957
- NLM abbreviation
- Dev Cell
- ISSN
- 1534-5807
- eISSN
- 1878-1551
- Publisher
- United States
- Grant note
- WT092825 / Wellcome Trust MC_PC_14105 / Medical Research Council MR/J000647/1 / Medical Research Council P30 CA086862 / NCI NIH HHS MR/K015664/1 / Medical Research Council R01 GM099481 / NIGMS NIH HHS
- Language
- English
- Date published
- 08/22/2016
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025301802771
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