Journal article
Fascin regulates chronic inflammation‐related human colon carcinogenesis by inhibiting cell anoikis
Proteomics (Weinheim), Vol.14(9), pp.1031-1041
05/2014
DOI: 10.1002/pmic.201300414
PMID: 24574163
Abstract
By a proteomics‐based approach, we identified an overexpression of fascin in colon adenocarcinoma cells (FPCKpP‐3) that developed from nontumorigenic human colonic adenoma cells (FPCK‐1–1) and were converted to tumorigenic by foreign‐body‐induced chronic inflammation in nude mice. Fascin overexpression was also observed in the tumors arising from rat intestinal epithelial cells (IEC 6) converted to tumorigenic in chronic inflammation which was induced in the same manner. Upregulation of fascin expression in FPCK‐1–1 cells by transfection with sense fascin cDNA converted the cells tumorigenic, whereas antisense fascin‐cDNA‐transfected FPCKpP‐3 cells reduced fascin expression and lost their tumor‐forming ability in vivo. The tumorigenic potential by fascin expression was consistent with their ability to survive and grow in the three‐dimensional multicellular spheroids. We found that resistance to anoikis (apoptotic cell death as a consequence of insufficient cell‐to‐substrate interactions), which is represented by the three‐dimensional growth of solid tumors in vivo, was regulated by fascin expression through caspase‐dependent apoptotic signals. From these, we demonstrate that fascin is a potent suppressor to caspase‐associated anoikis and accelerator of the conversion of colonic adenoma cells into adenocarcinoma cells by chronic inflammation.
Details
- Title: Subtitle
- Fascin regulates chronic inflammation‐related human colon carcinogenesis by inhibiting cell anoikis
- Creators
- Yusuke Kanda - Tottori UniversityTokuichi Kawaguchi - Japanese Foundation For Cancer ResearchYasuhiro Kuramitsu - Yamaguchi UniversityTakao Kitagawa - Yamaguchi UniversityTokushige Kobayashi - Kobayashi Dental Clinic Asahikawa JapanNorihiko Takahashi - Hokkaido UniversityHiroshi Tazawa - Okayama UniversityHasem Habelhah - University of IowaJun‐ichi Hamada - Hokkaido UniversityMasanobu Kobayashi - Health Sciences University of HokkaidoMio Hirahata - Tottori UniversityKunishige Onuma - Tottori UniversityMitsuhiko Osaki - Tottori UniversityKazuyuki Nakamura - Yamaguchi UniversityTomoyuki Kitagawa - Japanese Foundation For Cancer ResearchMasuo Hosokawa - Sapporo Cancer Seminar Foundation Sapporo JapanFutoshi Okada - Tottori University
- Resource Type
- Journal article
- Publication Details
- Proteomics (Weinheim), Vol.14(9), pp.1031-1041
- DOI
- 10.1002/pmic.201300414
- PMID
- 24574163
- NLM abbreviation
- Proteomics
- ISSN
- 1615-9853
- eISSN
- 1615-9861
- Number of pages
- 11
- Grant note
- Joint Research Program of the Institute for Genetic Medicine, Hokkaido University Japanese Ministry of Education, Culture, Sports, Science and Technology
- Language
- English
- Date published
- 05/2014
- Academic Unit
- Pathology
- Record Identifier
- 9984186546202771
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