Fascin regulates nuclear actin during Drosophila oogenesis

Daniel J Kelpsch; Christopher M Groen; Tiffany N Fagan; Sweta Sudhir; Tina L Tootle

doi:10.1091/mbc.E15-09-0634

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Fascin regulates nuclear actin during Drosophila oogenesis

Journal article

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Peer reviewed

Fascin regulates nuclear actin during Drosophila oogenesis

Daniel J Kelpsch, Christopher M Groen, Tiffany N Fagan, Sweta Sudhir and Tina L Tootle

Molecular biology of the cell, Vol.27(19), pp.2965-2979

10/01/2016

DOI: 10.1091/mbc.E15-09-0634

PMCID: PMC5042582

PMID: 27535426

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Abstract

Drosophila oogenesis provides a developmental system with which to study nuclear actin. During Stages 5-9, nuclear actin levels are high in the oocyte and exhibit variation within the nurse cells. Cofilin and Profilin, which regulate the nuclear import and export of actin, also localize to the nuclei. Expression of GFP-tagged Actin results in nuclear actin rod formation. These findings indicate that nuclear actin must be tightly regulated during oogenesis. One factor mediating this regulation is Fascin. Overexpression of Fascin enhances nuclear GFP-Actin rod formation, and Fascin colocalizes with the rods. Loss of Fascin reduces, whereas overexpression of Fascin increases, the frequency of nurse cells with high levels of nuclear actin, but neither alters the overall nuclear level of actin within the ovary. These data suggest that Fascin regulates the ability of specific cells to accumulate nuclear actin. Evidence indicates that Fascin positively regulates nuclear actin through Cofilin. Loss of Fascin results in decreased nuclear Cofilin. In addition, Fascin and Cofilin genetically interact, as double heterozygotes exhibit a reduction in the number of nurse cells with high nuclear actin levels. These findings are likely applicable beyond Drosophila follicle development, as the localization and functions of Fascin and the mechanisms regulating nuclear actin are widely conserved.

Oocytes - metabolism

Oogenesis - physiology

Actins - metabolism

Drosophila Proteins - metabolism

Actin Depolymerizing Factors - metabolism

Carrier Proteins - genetics

Animals

Carrier Proteins - metabolism

Cell Nucleus - metabolism

Female

Microfilament Proteins - metabolism

Drosophila - metabolism

Microfilament Proteins - genetics

Ovary - metabolism

Details

Title: Subtitle: Fascin regulates nuclear actin during Drosophila oogenesis
Creators: Daniel J Kelpsch - Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242
Christopher M Groen - Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242
Tiffany N Fagan - Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242
Sweta Sudhir - Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242
Tina L Tootle - Anatomy and Cell Biology, University of Iowa Carver College of Medicine, Iowa City, IA 52242 tina-tootle@uiowa.edu
Resource Type: Journal article
Publication Details: Molecular biology of the cell, Vol.27(19), pp.2965-2979
DOI: 10.1091/mbc.E15-09-0634
PMID: 27535426
PMCID: PMC5042582
NLM abbreviation: Mol Biol Cell
ISSN: 1059-1524
eISSN: 1939-4586
Publisher: American Society for Cell Biology; United States
Grant note: R01 GM116885 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS T32 NS045549 / NINDS NIH HHS
Language: English
Date published: 10/01/2016
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984025457602771

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