Journal article
Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivo
Developmental dynamics, Vol.249(8), pp.961-982
08/2020
DOI: 10.1002/dvdy.186
PMCID: PMC8900984
PMID: 32352613
Abstract
Background
The actin bundling protein Fascin is essential for developmental cell migrations and promotes cancer metastasis. In addition to bundling actin, Fascin has several actin‐independent roles; how these other functions contribute to cell migration remains unclear. Border cell migration during Drosophila oogenesis provides an excellent model to study Fascin's various roles during invasive, collective cell migration.
Results
On‐time border cell migration during Stage 9 requires Fascin (Drosophila Singed). Fascin functions not only within the migrating border cells, but also within the nurse cells, the substrate for this migration. Fascin genetically interacts with the actin elongation factor Enabled to promote on‐time Stage 9 migration and overexpression of Enabled suppresses the defects seen with loss of Fascin. Loss of Fascin results in increased, shorter and mislocalized protrusions during migration. Additionally, loss of Fascin inhibits border cell delamination and increases E‐Cadherin (Drosophila Shotgun) adhesions on both the border cells and nurse cells.
Conclusions
Overall, Fascin promotes on‐time border cell migration during Stage 9 and contributes to multiple aspects of this invasive, collective cell migration, including both protrusion dynamics and delamination. These findings have implications beyond Drosophila, as border cell migration has emerged as a model to study mechanisms mediating cancer metastasis.
Key Findings
Fascin functions in the migrating border cells and their substrate to regulate on‐time border cell migration during Stage 9 of Drosophila oogenesis.
Fascin genetically interacts with Enabled and regulates protrusion dynamics to promote migration.
Fascin limits E‐Cadherin membrane localization on the border cells and surrounding nurse cells, and regulates delamination.
Details
- Title: Subtitle
- Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivo
- Creators
- Maureen C. Lamb - University of IowaKelsey K. Anliker - Roy J. and Lucille A. Carver College of MedicineTina L. Tootle - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Developmental dynamics, Vol.249(8), pp.961-982
- DOI
- 10.1002/dvdy.186
- PMID
- 32352613
- PMCID
- PMC8900984
- NLM abbreviation
- Dev Dyn
- ISSN
- 1058-8388
- eISSN
- 1097-0177
- Publisher
- John Wiley & Sons, Inc
- Number of pages
- 22
- Grant note
- University of Iowa National Institute of General Medical Sciences (R01GM116885) National Institutes of Health (R01GM116885)
- Language
- English
- Date published
- 08/2020
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284457302771
Metrics
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