Journal article
Fast axonal diffusion of 3000 molecular weight dextran amines
Journal of neuroscience methods, Vol.50(1), pp.95-103
1993
DOI: 10.1016/0165-0270(93)90060-5
PMID: 7506342
Abstract
The distances of anterograde and retrograde axonal movement per hour were examined for dextran amines of 3000, 10,000 and 40,000 molecular weights (MW) conjugated to different fluorochromes (Cascade blue, fluorescein, tetramethylrhodamine, Texas red) or to biotin. Lateral line nerves of
Xenopus laevis tadpoles were used as an easily accessible test system. Only 10,000 and 3000 MW dextran amines underwent significant anterograde and retrograde movement. Dextrans of 3000 MW progressed about twice as far (2 mm/h at 22°C) than 10,000 MW dextrans. Dextrans conjugated to different fluorochromes or to biotin did not show differences in their distance covered. Tracers traveled over the same distance in amphibians pre-treated with either 1 μM colchicine for 2–6 h or 10 μg/ml nocodazole for 5 h prior to the application to depolymerize microtubules needed for active transport. This suggests that diffusion is the major mechanism of movement for dextran amines over short distances. In the developing
Xenopus retina, 3000 MW dextrans traveled from the optic disc as far away as the ora serrata within 1 h. In mouse embryos, chicken embryos and larval lampreys dextran amines progress within 1 h from cut peripheral nerves through efferent and afferent tracts. These data show that 3000 MW dextrans can be used in much the same way as 10,000 MW dextrans, but label neuronal profiles in a shorter time in a wide variety of species.
Details
- Title: Subtitle
- Fast axonal diffusion of 3000 molecular weight dextran amines
- Creators
- B Fritzsch - Department of Biomedical Sciences, Creighton University, Omaha, NE 68178 USA
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience methods, Vol.50(1), pp.95-103
- DOI
- 10.1016/0165-0270(93)90060-5
- PMID
- 7506342
- NLM abbreviation
- J Neurosci Methods
- ISSN
- 0165-0270
- eISSN
- 1872-678X
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1993
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070776602771
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