Fast synaptic vesicle reuse slows the rate of synaptic depression in the CA1 region of hippocampus

Mert Ertunc; Yildirim Sara; ChiHye Chung; Deniz Atasoy; Tuhin Virmani; Ege T Kavalali

doi:10.1523/JNEUROSCI.4051-06.2007

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Fast synaptic vesicle reuse slows the rate of synaptic depression in the CA1 region of hippocampus

Journal article

Open access

Peer reviewed

Fast synaptic vesicle reuse slows the rate of synaptic depression in the CA1 region of hippocampus

Mert Ertunc, Yildirim Sara, ChiHye Chung, Deniz Atasoy, Tuhin Virmani and Ege T Kavalali

The Journal of neuroscience, Vol.27(2), pp.341-354

01/10/2007

DOI: 10.1523/JNEUROSCI.4051-06.2007

PMCID: PMC6672081

PMID: 17215395

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Abstract

During short-term synaptic depression, neurotransmission rapidly decreases in response to repetitive action potential firing. Here, by blocking the vacuolar ATPase, alkalinizing the extracellular pH, or exposing hippocampal slices to pH buffers, we impaired neurotransmitter refilling, and electrophysiologically tested the role of vesicle reuse in synaptic depression. Under all conditions, synapses onto hippocampal CA1 pyramidal cells showed faster depression with increasing stimulation frequencies. At 20 Hz, compromising neurotransmitter refilling increased depression within 300 ms reaching completion within 2 s, suggesting a minimal contribution of reserve vesicles to neurotransmission. In contrast, at 1 Hz, depression emerged gradually and became significant within 100 s. Moreover, the depression induced by pH buffers was reversible with a similar frequency dependence, suggesting that the frequency-dependent increase in depression was caused by impairment of rapid synaptic vesicle reuse. These results indicate that synaptic vesicle trafficking impacts the kinetics of short-term synaptic plasticity at an extremely rapid time scale.

Long-Term Synaptic Depression - physiology

Animals

Synapses - physiology

Rats

Reaction Time - physiology

Hippocampus - physiology

Rats, Sprague-Dawley

Synaptic Vesicles - physiology

Details

Title: Subtitle: Fast synaptic vesicle reuse slows the rate of synaptic depression in the CA1 region of hippocampus
Creators: Mert Ertunc - Center for Basic Neuroscience and Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390-9111, USA
Yildirim Sara
ChiHye Chung
Deniz Atasoy
Tuhin Virmani
Ege T Kavalali
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.27(2), pp.341-354
Publisher: United States
DOI: 10.1523/JNEUROSCI.4051-06.2007
PMID: 17215395
PMCID: PMC6672081
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: MH068437 / NIMH NIH HHS
Language: English
Date published: 01/10/2007
Academic Unit: Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
Record Identifier: 9984040232602771

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