Journal article
Fast transport of neurofilament protein along microtubules in squid axoplasm
Journal of cell science, Vol.113(22), pp.3939-3946
11/2000
DOI: 10.1242/jcs.113.22.3939
PMID: 11058081
Abstract
Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal proteins, throughout neurons.
Details
- Title: Subtitle
- Fast transport of neurofilament protein along microtubules in squid axoplasm
- Creators
- Veena Prahlad - University of Iowa, Iowa Neuroscience InstituteBrian T HelfandGeorge M LangfordRon D ValeRobert D Goldman
- Resource Type
- Journal article
- Publication Details
- Journal of cell science, Vol.113(22), pp.3939-3946
- DOI
- 10.1242/jcs.113.22.3939
- PMID
- 11058081
- ISSN
- 0021-9533
- eISSN
- 1477-9137
- Language
- English
- Date published
- 11/2000
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984071688602771
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