Journal article
Fasting-induced reductions in cardiovascular and metabolic variables occur sooner in obese versus lean mice
Experimental biology and medicine (Maywood, N.J.), Vol.235(12), pp.1489-1497
12/2010
DOI: 10.1258/ebm.2010.010171
PMID: 21127345
Abstract
It is not uncommon for laboratory animals to be fasted prior to experimentation. Fasting evokes marked reductions in heart rate (HR), blood pressure (BP), heat production and oxygen consumption (VO2) in rodents. Mice with diet-induced obesity exhibit elevated HR and BP, and lower VO2 and heat production in the fed condition versus their lean counterparts. It is unknown whether body composition alters the tempo of response to fasting. We tested the hypothesis that cardiovascular and metabolic responses to fasting are delayed in obese versus lean male C57BL/6J mice. In the fed condition, mice that consumed high-fat (HF, 45% fat) chow for 98 ± 5 days had elevated (P < 0.05) body fat percentage (DEXA), serum leptin (ELISA), HR and BP (72-h biotelemetry), and lower (P < 0.05) heat production and VO2 (72-h metabolic chamber) versus animals that consumed standard chow (CON, 10% fat; n = 16 per group). HR, BP, VO2, heat production and serum leptin decreased (all P < 0.05) in response to a 16-h fast (16:00–08:00 h) in both groups. Although the overall fold changes in cardiovascular and metabolic parameters were similar in magnitude among animals, fasting-induced reductions in cardiovascular and metabolic variables occurred ∼4 and ∼7 h earlier (P < 0.05), respectively, in HF versus CON mice. These findings indicate that while metabolic and cardiovascular stress evoked by a 16-h fast at 22°C is not different between HF and CON mice, fasting-induced responses occur sooner in obese animals.
Details
- Title: Subtitle
- Fasting-induced reductions in cardiovascular and metabolic variables occur sooner in obese versus lean mice
- Creators
- Jason M Tanner - College of Health, University of Utah, Salt Lake City, UT 84132, Division of Endocrinology, Metabolism and DiabetesDevin T Kearns - College of Health, University of Utah, Salt Lake City, UT 84132Bum Jun Kim - Division of Endocrinology, Metabolism and DiabetesCrystal Sloan - Division of Endocrinology, Metabolism and Diabetes, Program in Molecular MedicineZhanjun Jia - Division of Nephrology and Hypertension, University of Utah School of Medicine, Salt Lake City, UT 84112, USATianxin Yang - Division of Nephrology and Hypertension, University of Utah School of Medicine, Salt Lake City, UT 84112, USAE Dale Abel - Division of Endocrinology, Metabolism and Diabetes, Program in Molecular MedicineJ David Symons - College of Health, University of Utah, Salt Lake City, UT 84132, Division of Endocrinology, Metabolism and Diabetes
- Resource Type
- Journal article
- Publication Details
- Experimental biology and medicine (Maywood, N.J.), Vol.235(12), pp.1489-1497
- DOI
- 10.1258/ebm.2010.010171
- PMID
- 21127345
- ISSN
- 1535-3702
- eISSN
- 1535-3699
- Language
- English
- Date published
- 12/2010
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984025258402771
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