Journal article
Fatty acid synthase expression during peripheral nervous system myelination
Brain research. Molecular brain research., Vol.101(1), pp.52-58
2002
DOI: 10.1016/S0169-328X(02)00161-4
PMID: 12007831
Abstract
The expression of fatty acid synthase (FAS) in rat and mouse sciatic nerves during postnatal development was investigated. FAS activity was not sensitive to the nutritional status of the animals. During development, the specific activity of FAS was low in rat and mouse nerves immediately after birth. Then, there was a steady increase in the activity (8- to 10-fold) which reached a maximal level around postnatal day 11, plateaued till day 32, and decreased to reach 30% of the maximum at day 80. A similar developmental profile was obtained when the amount of FAS protein was quantified, thus suggesting that the variations in activity observed during sciatic nerve development are mainly due to variations in FAS protein content. Northern blot analysis showed that the mRNA levels for FAS parallels those of the ceramide galactosyl transferase (CGT) during mouse sciatic nerve development and in a rat demyelination-nerve regeneration model. In addition, we measured FAS expression in the sciatic nerves of the trembler mutant, which is a mouse model of PNS dysmyelination. In 20-day-old trembler nerves, FAS specific activity, protein amount and mRNA levels represented only 25% of the normal values. Altogether, our data indicate that FAS expression is linked to the PNS myelination process, and that the main regulation occurs at the level of the gene expression.
Details
- Title: Subtitle
- Fatty acid synthase expression during peripheral nervous system myelination
- Creators
- Jérôme Salles - Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, case 92, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, FranceFrançoise Sargueil - Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, case 92, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, FranceAnja Knoll-Gellida - Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, case 92, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, FranceLee A Witters - Endocrine-Metabolism Division, Departments of Medicine and Biochemistry, Dartmouth Medical School, Hanover, NH, USAMichael Shy - Department of Neurology, Wayne State University School of Medicine, Detroit, MI, USAHuiyuan Jiang - Department of Neurology, Wayne State University School of Medicine, Detroit, MI, USAClaude Cassagne - Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, case 92, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, FranceBertrand Garbay - Laboratoire de Biogenèse Membranaire, UMR-CNRS 5544, case 92, Université Victor Segalen Bordeaux 2, 146 rue Léo Saignat, 33076 Bordeaux cedex, France
- Resource Type
- Journal article
- Publication Details
- Brain research. Molecular brain research., Vol.101(1), pp.52-58
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0169-328X(02)00161-4
- PMID
- 12007831
- ISSN
- 0169-328X
- eISSN
- 1872-6941
- Language
- English
- Date published
- 2002
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
- Record Identifier
- 9984020709602771
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