Fentanyl vapor self-administration model in mice to study opioid addiction

K Moussawi; M. M Ortiz; S. C Gantz; B. J Tunstall; R. C. N Marchette; A Bonci; G. F Koob; L. F Vendruscolo

doi:10.1126/sciadv.abc0413

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Fentanyl vapor self-administration model in mice to study opioid addiction

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Peer reviewed

Fentanyl vapor self-administration model in mice to study opioid addiction

K Moussawi, M. M Ortiz, S. C Gantz, B. J Tunstall, R. C. N Marchette, A Bonci, G. F Koob and L. F Vendruscolo

Science advances, Vol.6(32), pp.eabc0413-eabc0413

08/2020

DOI: 10.1126/sciadv.abc0413

PMID: 32821843

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Abstract

Intravenous drug self-administration is considered the “gold standard” model to investigate the neurobiology of drug addiction in rodents. However, its use in mice is limited by frequent complications of intravenous catheterization. Given the many advantages of using mice in biomedical research, we developed a noninvasive mouse model of opioid self-administration using vaporized fentanyl. Mice readily self-administered fentanyl vapor, titrated their drug intake, and exhibited addiction-like behaviors, including escalation of drug intake, somatic signs of withdrawal, drug intake despite punishment, and reinstatement of drug seeking. Electrophysiological recordings from ventral tegmental area dopamine neurons showed a lower amplitude of GABAB receptor–dependent currents during protracted abstinence from fentanyl vapor self-administration. This mouse model of fentanyl self-administration recapitulates key features of opioid addiction, overcomes limitations of the intravenous model, and allows investigation of the neurobiology of opioid addiction in unprecedented ways.

Details

Title: Subtitle: Fentanyl vapor self-administration model in mice to study opioid addiction
Creators: K Moussawi - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA., Neurology Department, Johns Hopkins Medicine, Baltimore, MD, USA
M. M Ortiz - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA
S. C Gantz - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA., Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA, USA
B. J Tunstall - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA
R. C. N Marchette - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA
A Bonci - Global Institutes on Addictions, Miami, FL, USA
G. F Koob - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA
L. F Vendruscolo - Intramural Research Program, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA
Resource Type: Journal article
Publication Details: Science advances, Vol.6(32), pp.eabc0413-eabc0413
DOI: 10.1126/sciadv.abc0413
PMID: 32821843
NLM abbreviation: Sci Adv
ISSN: 2375-2548
eISSN: 2375-2548
Grant note: DOI: 10.13039/100000026, name: National Institute on Drug Abuse, award: DA048085; DOI: 10.13039/100000026, name: National Institute on Drug Abuse, award: DA048530
Language: English
Date published: 08/2020
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute
Record Identifier: 9984065367702771

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