Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines

B Fritzsch; M A Christensen; D H Nichols

doi:10.1002/neu.480241104

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Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines

Journal article

Peer reviewed

Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines

B Fritzsch, M A Christensen and D H Nichols

Journal of neurobiology, Vol.24(11), pp.1481-1499

11/1993

DOI: 10.1002/neu.480241104

PMID: 7506749

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Abstract

The differentiation of facial motoneurons and inner ear (octaval) efferents was examined in chicken embryos by applying DiI or dextran amines to the cut VII/VIII nerve (peripheral label) or to the basal/floor plate of rhombomeres 4/5 (central label). Central labeling found axons of these efferent neurons to leave the brain as early as 2.5 days of incubation. Peripheral labeling identified cell bodies ipsilaterally in rhombomeres 4 and 5 at 2.5 days. Central labeling at 3.5 days showed these fibers to have fully segregated into separate pathways to the facial nerve and the inner ear and that the octaval efferent axons had reached the otocyst wall. By 3.5 days many peripherally labeled octaval efferent somata were found in the floor plate and by 5 days they were found bilaterally. At 6 days, selective peripheral labeling of either the VIIth or VIIIth nerve showed that the contralateral population consisted of octaval efferents and central label applied to the floor plate of rhombomeres 4/5 identified fibers that entered the octaval nerve via the facial root and entered the vestibular sensory epithelia. Together these data suggest an initial mingling of two different motoneuron populations (facial and octaval) in rhombomeres 4/5 and a subsequent segregation by differential migration. Our data also find a much earlier arrival of octaval efferent axons at the otic vesicle than previously described and suggest a contralateral migration of many octaval efferents beginning shortly after their axons reach the facial nerve root.

Cell Differentiation

Peripheral Nervous System - physiology

Ear, Inner - innervation

Efferent Pathways - ultrastructure

Motor Neurons - physiology

Efferent Pathways - physiology

Efferent Pathways - embryology

Biotin

Facial Nerve - cytology

Peripheral Nervous System - embryology

Central Nervous System - embryology

Chick Embryo

Peripheral Nervous System - cytology

Carbocyanines

Central Nervous System - physiology

Animals

Dextrans

Nerve Fibers - physiology

Central Nervous System - cytology

Histocytochemistry

Nerve Fibers - ultrastructure

Facial Nerve - embryology

Details

Title: Subtitle: Fiber pathways and positional changes in efferent perikarya of 2.5- to 7-day chick embryos as revealed with DiI and dextran amines
Creators: B Fritzsch - Creighton University, Department of Biomedical Sciences, Omaha, Nebraska 68178
M A Christensen
D H Nichols
Resource Type: Journal article
Publication Details: Journal of neurobiology, Vol.24(11), pp.1481-1499
DOI: 10.1002/neu.480241104
PMID: 7506749
NLM abbreviation: J Neurobiol
ISSN: 0022-3034
eISSN: 1097-4695
Publisher: Wiley; United States
Grant note: P50 DC00215-09 / NIDCD NIH HHS
Language: English
Date published: 11/1993
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070007302771

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