Journal article
Fibronectin and Integrin α5 play overlapping and independent roles in regulating the development of pharyngeal endoderm and cartilage
Developmental biology, Vol.489, pp.122-133
09/2022
DOI: 10.1016/j.ydbio.2022.06.010
Abstract
Craniofacial skeletal elements are derived from cranial neural crest cells (CNCCs), which migrate along discrete paths and populate distinct pharyngeal arches, structures that are separated by the neighboring endodermal pouches (EPs). Interactions between the CNCCs and the endoderm are critical for proper craniofacial development. In zebrafish, integrin α5 (Itga5) functions in the endoderm to regulate formation of specifically the first EP (EP1) and the development of the hyoid cartilage. Here we show that fibronectin (Fn), a major component of the extracellular matrix (ECM), is also required for these developmental processes, and that the penetrance of defects in mutants is temperature-dependent. fn1a−/− embryos exhibited defects that are similar to, but much more severe than, those of itga5−/− embryos, and a loss of integrin av (itgav) function enhanced both endoderm and cartilage defects in itga5−/− embryos, suggesting that Itga5 and Itgav cooperate to transmit signals from Fn to regulate the development of endoderm and cartilage. Whereas the endodermal defects in itga5; itga5v−/− double mutant embryos were comparable to those of fn1a−/− mutants, the cartilage defects were much milder. Furthermore, Fn assembly was detected in migrating CNCCs, and the epithelial organization and differentiation of CNCC-derived arches were impaired in fn1a−/− embryos, indicating that Fn1 exerts functions in arch development that are independent of Itga5 and Itgav. Additionally, reduction of itga5 function in fn1a−/− embryos led to profound defects in body axis elongation, as well as in endoderm and cartilage formation, suggesting that other ECM proteins signal through Itga5 to regulate development of the endoderm and cartilage. Thus, our studies reveal that Fn1a and Itga5 have both overlapping and independent functions in regulating development of the pharyngeal endoderm and cartilage.
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•Fn1a is required for morphogenesis of the pharyngeal endoderm and the development of cartilage.•Integrin α5 interacts with integrin αv to regulate endoderm morphogenesis and cartilage formation.•Fn1a likely functions through integrin α5 and integrin αv to orchestrate endoderm development.•Fn1a is critical for the formation of cranial neural crest cell-derived arches.
Details
- Title: Subtitle
- Fibronectin and Integrin α5 play overlapping and independent roles in regulating the development of pharyngeal endoderm and cartilage
- Creators
- Yuanyuan Gao - Roy J. and Lucille A. Carver College of MedicineBo HuRickcardo FloresHuaping XieFang Lin
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.489, pp.122-133
- DOI
- 10.1016/j.ydbio.2022.06.010
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Grant note
- DOI: 10.13039/100000001, name: National Science Foundation, award: 2054512, IOS-1354457; DOI: 10.13039/100003187, name: NSF
- Language
- English
- Date published
- 09/2022
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984288637202771
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