Journal article
Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta
The Journal of biological chemistry, Vol.274(52), pp.36835-36838
12/24/1999
DOI: 10.1074/jbc.274.52.36835
PMID: 10601233
Abstract
The yeast RAD30 gene functions in error-free replication of UV-damaged DNA, and RAD30 encodes a DNA polymerase, pol eta, that has the ability to efficiently and correctly replicate past a cis-syn-thymine-thymine dimer in template DNA. To better understand the role of pol eta in damage bypass, we examined its fidelity and processivity on nondamaged DNA templates. Steady-state kinetic analyses of deoxynucleotide incorporation indicate that pol eta has a low fidelity, misincorporating deoxynucleotides with a frequency of about 10(-2) to 10(-3). Also pol eta has a low processivity, incorporating only a few nucleotides before dissociating. We suggest that pol eta's low fidelity reflects a flexibility in its active site rendering it more tolerant of DNA damage, while its low processivity limits its activity to reduce errors.
Details
- Title: Subtitle
- Fidelity and processivity of Saccharomyces cerevisiae DNA polymerase eta
- Creators
- M Todd Washington - Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston, Texas 77555-1061, USARobert E JohnsonSatya PrakashLouise Prakash
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.274(52), pp.36835-36838
- DOI
- 10.1074/jbc.274.52.36835
- PMID
- 10601233
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- United States
- Grant note
- GM19261 / NIGMS NIH HHS
- Language
- English
- Date published
- 12/24/1999
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024530902771
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