Journal article
Field evaluation of endotoxin air sampling assay methods
American Industrial Hygiene Association journal, Vol.58(11), pp.792-799
1997
DOI: 10.1080/15428119791012298
PMID: 9373925
Abstract
This study tested the importance of filter media, extraction and assay protocol, and bioaerosol source on the determination of endotoxin under field conditions in swine and poultry confinement buildings. Multiple simultaneous air samples were collected using glass fiber (GF) and polycarbonate (PC) filters, and these were assayed using two methods in two separate laboratories: an endpoint chromogenic Limulus amebocyte lysate (LAL) assay (QCL) performed in water and a kinetic chromogenic LAL assay (KQCL) performed in buffer with resistant-parallel line estimation analysis (KLARE). In addition, two aqueous filter extraction methods were compared in the QCL assay: 120 min extraction at 22°C with vigorous shaking and 30 min extraction at 68°C with gentle rocking. These extraction methods yielded endotoxin activities that were not significantly different and were very highly correlated. Reproducibility of endotoxin determinations from duplicate air sampling filters was very high (Cronbach α all > 0.94). When analyzed by the QCL method GF filters yielded significantly higher endotoxin activity than PC filters. QCL and KLARE methods gave similar estimates for endotoxin activity from PC filters; however, GF filters analyzed by the QCL method yielded significantly higher endotoxin activity estimates, suggesting enhancement of the QCL assay or inhibition of the KLARE assay with GF filters. Correlation between QCL-GF and QCL-PC was high (r = 0.98) while that between KLARE-GF and KLARE-PC was moderate (r = 0.68). Analysis of variance demonstrated that assay methodology, filter-type, barn-type, and interactions between assay and filter-type and between assay and barn-type were important factors influencing endotoxin exposure assessment.
Details
- Title: Subtitle
- Field evaluation of endotoxin air sampling assay methods
- Creators
- Peter S Thorne - University of Iowa, Occupational and Environmental HealthStephen J. Reynolds - Department of Preventive Medicine & Environmental Health, The University of Iowa, 100 Oakdale Campus, #176 IREH, Iowa City, IA 52242-5000, United StatesDonald K Milton - Department of Environmental Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, United StatesPeggy D Bloebaum - Department of Preventive Medicine & Environmental Health, The University of Iowa, 100 Oakdale Campus, #176 IREH, Iowa City, IA 52242-5000, United StatesXiaoji Zhang - University of IowaPaul Whitten - Department of Preventive Medicine & Environmental Health, The University of Iowa, 100 Oakdale Campus, #176 IREH, Iowa City, IA 52242-5000, United StatesLeon F. Burmeister - Department of Preventive Medicine & Environmental Health, The University of Iowa, 100 Oakdale Campus, #176 IREH, Iowa City, IA 52242-5000, United States
- Resource Type
- Journal article
- Publication Details
- American Industrial Hygiene Association journal, Vol.58(11), pp.792-799
- Publisher
- American Industrial Hygiene Association
- DOI
- 10.1080/15428119791012298
- PMID
- 9373925
- ISSN
- 0002-8894
- eISSN
- 2163-369X
- Language
- English
- Date published
- 1997
- Academic Unit
- Civil and Environmental Engineering; Occupational and Environmental Health
- Record Identifier
- 9983557694402771
Metrics
28 Record Views