Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Meng Yang; Alexandra Brand; Thyagarajan Srikantha; Karla J Daniels; David R Soll; Neil A R Gow

doi:10.1128/EC.00145-10

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Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Journal article

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Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans

Meng Yang, Alexandra Brand, Thyagarajan Srikantha, Karla J Daniels, David R Soll and Neil A R Gow

Eukaryotic cell, Vol.10(3), pp.435-444

03/2011

DOI: 10.1128/EC.00145-10

PMCID: PMC3067472

PMID: 21216943

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Abstract

Few mating-regulated genes have been characterized in Candida albicans. C. albicans FIG1 (CaFIG1) is a fungus-specific and mating-induced gene encoding a putative 4-transmembrane domain protein that shares sequence similarities with members of the claudin superfamily. In Saccharomyces cerevisiae, Fig1 is required for shmoo fusion and is upregulated in response to mating pheromones. Expression of CaFIG1 was also strongly activated in the presence of cells of the opposite mating type. CaFig1-green fluorescent protein (GFP) was visible only during the mating response, when it localized predominantly to the plasma membrane and perinuclear zone in mating projections and daughter cells. At the plasma membrane, CaFig1-GFP was visualized as discontinuous zones, but the distribution of perinuclear CaFig1-GFP was homogeneous. Exposure to pheromone induced a 5-fold increase in Ca(2+) uptake in mating-competent opaque cells. Uptake was reduced substantially in the fig1Δ null mutant. CaFig1 is therefore involved in Ca(2+) influx and localizes to membranes that are destined to undergo fusion during mating.

Fungal Proteins - chemistry

Gene Expression Regulation, Fungal

Amino Acid Sequence

Calcium - metabolism

Molecular Sequence Data

Cell Membrane - genetics

Candida albicans - metabolism

Fungal Proteins - genetics

Pheromones - metabolism

Candida albicans - cytology

Protein Transport

Candida albicans - genetics

Cell Membrane - chemistry

Sequence Alignment

Biological Transport

Candida albicans - chemistry

Cell Membrane - metabolism

Genes, Mating Type, Fungal

Fungal Proteins - metabolism

Details

Title: Subtitle: Fig1 facilitates calcium influx and localizes to membranes destined to undergo fusion during mating in Candida albicans
Creators: Meng Yang - School of Life Sciences & Medicine, Institute of Medical Science, University of Aberdeen, Foresterhill, Aberdeen, United Kingdom. n.gow@abdn.ac.uk
Alexandra Brand
Thyagarajan Srikantha
Karla J Daniels
David R Soll
Neil A R Gow
Resource Type: Journal article
Publication Details: Eukaryotic cell, Vol.10(3), pp.435-444
DOI: 10.1128/EC.00145-10
PMID: 21216943
PMCID: PMC3067472
NLM abbreviation: Eukaryot Cell
ISSN: 1535-9778
eISSN: 1535-9786
Publisher: American Society for Microbiology; United States
Grant note: Wellcome Trust BB/E008372/1 / Biotechnology and Biological Sciences Research Council
Language: English
Date published: 03/2011
Academic Unit: Biology
Record Identifier: 9983992058502771

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