Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae

D B Hornick; B L Allen; M A Horn; S Clegg

doi:10.1128/jcm.29.9.1795-1800.1991

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Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae

Journal article

Open access

Peer reviewed

Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae

D B Hornick, B L Allen, M A Horn and S Clegg

Journal of clinical microbiology, Vol.29(9), pp.1795-1800

09/1991

DOI: 10.1128/jcm.29.9.1795-1800.1991

PMCID: PMC270213

PMID: 1685495

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Abstract

Bacterial attachment is believed to be an early step in gram-negative nosocomial pneumonia. The frequency of fimbria-associated adhesins among respiratory pathogens has not been studied in detail. In this study isolates belonging to the family Enterobacteriaceae, prospectively obtained from intensive care unit patients who were suspected of having nosocomial pneumonia, were examined for fimbria-associated adhesins. Type 3, P, type 1, and other fimbrial phenotypes were identified by specific hemagglutination and electron microscopy. The Klebsiella type 3 fimbrial phenotype was further characterized by using a monoclonal antibody. Also, both type 3 and Escherichia coli P fimbrial genotypes were detected by using DNA colony blot assays. The frequencies of genera or species isolated were as follows: Enterobacter (38.6%), Klebsiella (26.8%), Serratia (17.7%), E. coli (13%), and Proteus (5.2%). Isolates of Klebsiella oxytoca, K. pneumoniae, and Enterobacter cloacae most commonly possessed the type 3 fimbrial phenotype and genotype. The phenotype and genotype for E. coli P fimbriae (46.2 and 50%, respectively), a known pathogenic determinant in the urinary tract, were detected more frequently than expected. In addition, a previously unspecified hemagglutinin that was specific for porcine erythrocytes was almost uniformly expressed among isolates of Enterobacter aerogenes. Finally, the expression of the type 1 fimbrial phenotype was widely detected among the isolates tested but notably absent among K. oxytoca and Proteus mirabilis isolates. The frequency of the various fimbrial types identified suggests a role for these bacterial organelles in adherence to respiratory epithelia.

Phenotype

Plasmids

Enterobacteriaceae Infections - microbiology

Humans

Enterobacteriaceae - genetics

Enterobacteriaceae - isolation & purification

Fimbriae, Bacterial - ultrastructure

Pneumonia - microbiology

Respiratory System - microbiology

Microscopy, Electron

Bacterial Adhesion

Enterobacteriaceae - ultrastructure

Hemagglutination

Cross Infection - microbiology

Fimbriae, Bacterial - immunology

Details

Title: Subtitle: Fimbrial types among respiratory isolates belonging to the family Enterobacteriaceae
Creators: D B Hornick - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242
B L Allen
M A Horn
S Clegg
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.29(9), pp.1795-1800
DOI: 10.1128/jcm.29.9.1795-1800.1991
PMID: 1685495
PMCID: PMC270213
ISSN: 0095-1137
eISSN: 1098-660X
Grant note: GM07337 / NIGMS NIH HHS
Language: English
Date published: 09/1991
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Internal Medicine
Record Identifier: 9984094748902771

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