Journal article
Find and replace: editing human genome in pluripotent stem cells
Protein & cell, Vol.2(12), pp.950-956
12/2011
DOI: 10.1007/s13238-011-1132-0
PMCID: PMC4875250
PMID: 22173708
Abstract
Genetic manipulation of human pluripotent stem cells (hPSCs) provides a powerful tool for modeling diseases and developing future medicine. Recently a number of independent genome-editing techniques were developed, including plasmid, bacterial artificial chromosome, adeno-associated virus vector, zinc finger nuclease, transcription activator-like effecter nuclease, and helper-dependent adenoviral vector. Gene editing has been successfully employed in different aspects of stem cell research such as gene correction, mutation knock-in, and establishment of reporter cell lines (Raya et al., 2009; Howden et al., 2011; Li et al., 2011; Liu et al., 2011b; Papapetrou et al., 2011; Sebastiano et al., 2011; Soldner et al., 2011; Zou et al., 2011a). These techniques combined with the utility of hPSCs will significantly influence the area of regenerative medicine.
Details
- Title: Subtitle
- Find and replace: editing human genome in pluripotent stem cells
- Creators
- Huize Pan - Beijing, 100101 ChinaWeiqi Zhang - Beijing, 100101 ChinaWeizhou Zhang - San Diego, La Jolla, CA 92093 USAGuang-Hui Liu - Beijing, 100101 China
- Resource Type
- Journal article
- Publication Details
- Protein & cell, Vol.2(12), pp.950-956
- DOI
- 10.1007/s13238-011-1132-0
- PMID
- 22173708
- PMCID
- PMC4875250
- NLM abbreviation
- Protein Cell
- ISSN
- 1674-800X
- eISSN
- 1674-8018
- Publisher
- Higher Education Press
- Language
- English
- Date published
- 12/2011
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984083269802771
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