Journal article
Flanking AT base pairs affect the localization of monovalent cations in DNA A-tracts
Electrophoresis, Vol.45(5-6), pp.528-536
03/2024
DOI: 10.1002/elps.202300220
PMID: 38087830
Appears in UI Libraries Support Open Access
Abstract
Capillary electrophoresis has been used to measure the free solution mobilities of a series of 26-base pair (bp) DNA oligomers containing two phased A4T1in-tracts embedded in flanking sequences containing 0 to 11 additional AT bps. A random-sequence 26-bp oligomer with 12 isolated AT bps was used as the reference. Mobility ratios (A-tract/reference) were measured in background electrolytes (BGEs) containing mixtures of small monovalent cations and tetrabutylammonium (TBA
) or tetrapropylammonium (TPA
) ions. The mobility ratios observed in 0.3 M TBA
were >1.00, suggesting that the TBA
ions had formed electrostatic contact pairs with the AT bp in the reference and in the A-tract flanking sequences, decreasing the mobilities of both oligomers. The TBA-AT pairing interactions could be eliminated by increasing the concentration of small monovalent cations in the BGE. In 0.3 M TPA
, electrostatic contact pairs were formed with the AT bps in the flanking sequences and in the A-tracts. Interestingly, the shapes of the mobility ratio profiles observed for the A4T1in-tract oligomers depended on the total number of A + T residues in the oligomer.
Details
- Title: Subtitle
- Flanking AT base pairs affect the localization of monovalent cations in DNA A-tracts
- Creators
- Earle Stellwagen - University of Iowa, Biochemistry and Molecular BiologyNancy C Stellwagen - University of Iowa, Biochemistry and Molecular Biology
- Resource Type
- Journal article
- Publication Details
- Electrophoresis, Vol.45(5-6), pp.528-536
- Publisher
- Wiley
- DOI
- 10.1002/elps.202300220
- PMID
- 38087830
- ISSN
- 0173-0835
- eISSN
- 1522-2683
- Grant note
- National Institute of General Medical Sciences. Grant Number: GM61009
- Language
- English
- Date published
- 03/2024
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984573142402771
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