Journal article
Flexibility of PCNA-Protein Interface Accommodates Differential Binding Partners
PloS one, Vol.9(7), pp.e102481-e102481
07/18/2014
DOI: 10.1371/journal.pone.0102481
PMCID: PMC4103810
PMID: 25036435
Abstract
The expanding roles of PCNA in functional assembly of DNA replication and repair complexes motivated investigation of the structural and dynamic properties guiding specificity of PCNA-protein interactions. A series of biochemical and computational analyses were combined to evaluate the PIP Box recognition features impacting complex formation. The results indicate subtle differences in topological and molecular descriptors distinguishing both affinity and stoichiometry of binding among PCNA-peptide complexes through cooperative effects. These features were validated using peptide mimics of p85 alpha and Akt, two previously unreported PCNA binding partners. This study characterizes for the first time a reverse PIP Box interaction with PCNA. Small molecule ligand binding at the PIP Box interaction site confirmed the adaptive nature of the protein in dictating overall shape and implicates allosterism in transmitting biological effects.
Details
- Title: Subtitle
- Flexibility of PCNA-Protein Interface Accommodates Differential Binding Partners
- Creators
- Anthony M. Pedley - Purdue University West LafayetteMarkus A. Lill - Purdue University West LafayetteV. Jo Davisson - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.9(7), pp.e102481-e102481
- DOI
- 10.1371/journal.pone.0102481
- PMID
- 25036435
- PMCID
- PMC4103810
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 12
- Grant note
- W81XWH-10-1-0105; W81XWH-11-1-0050 / Office of the Assistant Secretary of Defense for Health Affairs through the Breast Cancer Research Program Purdue Center for Cancer Research
- Language
- English
- Date published
- 07/18/2014
- Academic Unit
- Biochemistry and Molecular Biology
- Record Identifier
- 9984771655802771
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