Journal article
Flow Cytometry Quantification of Transient Transfections in Mammalian Cells
Methods in molecular biology (Clifton, N.J.), Vol.2774, pp.153-176
2024
DOI: 10.1007/978-1-0716-3718-0_11
PMID: 38441764
Abstract
Flow cytometry is a powerful quantitative assay supporting high-throughput collection of single-cell data with a high dynamic range. For flow cytometry to yield reproducible data with a quantitative relationship to the underlying biology, however, requires that (1) appropriate process controls are collected along with experimental samples, (2) these process controls are used for unit calibration and quality control, and (3) data are analyzed using appropriate statistics. To this end, this chapter describes methods for quantitative flow cytometry through the addition of process controls and analyses, thereby enabling better development, modeling, and debugging of engineered biological organisms. The methods described here have specifically been developed in the context of transient transfections in mammalian cells but may in many cases be adaptable to other categories of transfection and other types of cells.
Details
- Title: Subtitle
- Flow Cytometry Quantification of Transient Transfections in Mammalian Cells
- Creators
- Jacob Beal - RTX
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.2774, pp.153-176
- DOI
- 10.1007/978-1-0716-3718-0_11
- PMID
- 38441764
- eISBN
- 1071637185; 9781071637180
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2024
- Academic Unit
- Electrical and Computer Engineering
- Record Identifier
- 9984627344702771
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