Journal article
Flow cytometric aberrancies in plasma cell myeloma and MGUS – correlation with laboratory parameters
Cytometry. Part B, Clinical cytometry, Vol.94(3), pp.500-508
05/2018
DOI: 10.1002/cyto.b.21624
PMCID: PMC6342462
PMID: 29316245
Abstract
Background
Multiparametric flow cytometry is a useful tool for diagnosis of plasma cell (PC) dyscrasias and assessment of minimal residual disease in plasma cell myeloma (PCM). However, the immunophenotypic differences between the clonal PCs of PCM and those of monoclonal gammopathy of undetermined significance (MGUS) as well as the correlation of these flow cytometric markers with pertinent laboratory parameters have not been evaluated.
Methods
We retrospectively identified all newly diagnosed treatment‐naive PCM and MGUS patients between 09/2014 and 06/2015 who underwent 10‐color flow‐cytometric evaluation: CD45, CD38, CD138, cKappa, cLambda, CD19, CD27, CD28, CD56, CD117. FACSDiva analysis was used to identify antigenic aberrancies and associations with pertinent laboratory parameters were evaluated.
Results
All cases demonstrated at least two aberrancies. There was a trend toward a greater number of aberrancies in PCM, with 68% showing >/= 4 aberrancies compared with 44% in MGUS (P = 0.11). The only marker more frequently aberrant in one disease class was CD19, aberrant in 68% of PCM and 25% of MGUS (P < 0.01). In PCM, significant associations were found for CD56 non‐aberrancy (P = 0.05) and the presence of amyloid and CD27 aberrancy and normal serum albumin (P = 0.05). In MGUS, CD117 expression was associated with normal hemoglobin (P = 0.03).
Conclusions
The PCs of PCM show a trend toward more antigenic aberrancy than those of MGUS. There is significant association between the antigenic profiles of PCM/MGUS and clinical parameters including amyloidosis, albumin level, and hemoglobin. © 2018 International Clinical Cytometry Society
Details
- Title: Subtitle
- Flow cytometric aberrancies in plasma cell myeloma and MGUS – correlation with laboratory parameters
- Creators
- Sarika Gupta - University of IowaNitin J Karandikar - University of IowaTimothy Ginader - University of IowaAndrew M Bellizzi - University of IowaCarol J Holman - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cytometry. Part B, Clinical cytometry, Vol.94(3), pp.500-508
- DOI
- 10.1002/cyto.b.21624
- PMID
- 29316245
- PMCID
- PMC6342462
- NLM abbreviation
- Cytometry B Clin Cytom
- ISSN
- 1552-4949
- eISSN
- 1552-4957
- Number of pages
- 9
- Language
- English
- Date published
- 05/2018
- Academic Unit
- Pathology
- Record Identifier
- 9984047715902771
Metrics
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