Journal article
Flow cytometric analysis of DNA content in human bladder tumors and irrigation fluids
Cancer, Vol.56(7), pp.1677-1681
10/01/1985
DOI: 10.1002/1097-0142(19851001)56:7<1677::AID-CNCR2820560734>3.0.CO;2-Y
PMID: 4027899
Abstract
Flow cytometry (FCM) was used to study the DNA distribution of 99 tumor biopsy specimens and 41 bladder irrigation samples from patients with transitional cell carcinoma of the bladder. For tumor biopssy and cystectomy specimens, the frequency of aneuploidy increased with advancing tumor stage and grade. All TO tumors were diploid. Twenty‐seven percent of T1, 71.4% of T2, and 75% of T3 and T4 tumors were aneuploid. All Grade I tumors were diploid. Thirty percent of Grade II and 76.9% of Grade III tumors were aneuploid. The frequency of aneuploidy of tumors in the early stages (Ta, T1) is similar to the incidence of subsequent progression by these tumors described in the literature. For irrigation fluids, the relationship between grade and stage and the frequency of aneuploidy was similar to the relationship seen with tumor specimens. All four patients with only carcinoma in situ had aneuploid cells in their irrigations. The comparison of FCM data of bladder biopsy and bladder irrigation from the same cystoscopic evaluation suggests adequate representation of tumor cells in the irrigation fluids for almost all cases. The authors conclude that DNA ploidy analysis by FCM appears useful in a clinically important group of patients with aneuploid superficial tumors of moderate or high grade. Bladder irrigation analysis appears useful in the follow‐up of patients with a history of carcinoma in situ and those with aneuploid tumors. Cancer 56: 1677‐1681, 1985.
Details
- Title: Subtitle
- Flow cytometric analysis of DNA content in human bladder tumors and irrigation fluids
- Creators
- Joseph L. Chin - Millard Fillmore Suburban HospitalRobert P. Huben - Millard Fillmore Suburban HospitalEnriquetta Nava - Roswell Park Cancer InstituteYoucef M. Rustum - Roswell Park Cancer InstituteJoseph M. GrecoJ. Edson Pontes - Millard Fillmore Suburban HospitalOskar S. Frankfurt - Department of Department of Urology, Millard Fillmore Hospital, Buffalo, New York
- Resource Type
- Journal article
- Publication Details
- Cancer, Vol.56(7), pp.1677-1681
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/1097-0142(19851001)56:7<1677::AID-CNCR2820560734>3.0.CO;2-Y
- PMID
- 4027899
- ISSN
- 0008-543X
- eISSN
- 1097-0142
- Number of pages
- 5
- Grant note
- NCI (CA21071) National Bladder Cancer Collaborative Group A, NC1 (CA15937)
- Language
- English
- Date published
- 10/01/1985
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359687302771
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