Flow cytometric features of angioimmunoblastic T-cell lymphoma

Weina Chen; Melissa V Kesler; Nitin J Karandikar; Robert W McKenna; Steven H Kroft

doi:10.1002/cyto.b.20107

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Flow cytometric features of angioimmunoblastic T-cell lymphoma

Journal article

Open access

Flow cytometric features of angioimmunoblastic T-cell lymphoma

Weina Chen, Melissa V Kesler, Nitin J Karandikar, Robert W McKenna and Steven H Kroft

Cytometry. Part B, Clinical cytometry, Vol.70(3), pp.142-148

05/2006

DOI: 10.1002/cyto.b.20107

PMID: 16572417

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Abstract

The immunophenotypic features of angioimmunoblastic T-cell lymphoma (AILT) have not been well described. We retrospectively reviewed our institutional experience with the flow cytometric features of 16 cases of AILT. Multiparameter flow cytometry was able to identify a distinct population of immunophenotypically aberrant T cells in 15 of 16 cases. In 13 lymph node specimens, the neoplastic cells ranged from 1.9 to 87% (median 23%) of cells. The ratio of reactive to neoplastic T cells ranged from 0.01 to 20 (median 1.5); reactive T cells outnumbered neoplastic in 9/13 (69%) cases. The neoplastic populations expressed CD2, CD4, CD5, and CD45RO in all cases, lacked expression of CD8 and CD56 in all cases, and showed negative or dim surface CD3 in most cases. CD10 was expressed by the neoplastic populations in 11 of 14 cases at diagnosis; in 3 of these 11 only a subpopulation of the neoplastic cells was CD10(+). CD10 tended to be absent on neoplastic cells in staging bone marrows. The neoplastic population in all but one of the 15 positive cases possessed multiple immunophenotypic abnormalities and these were generally retained during the follow-up analyses of several cases. These results indicate the potential utility of flow cytometry in the diagnosis and follow-up of AILT.

Flow Cytometry - methods

Lymphoma, T-Cell - immunology

Lymph Nodes - pathology

Follow-Up Studies

Humans

Middle Aged

Male

Antigens, CD - analysis

Leukocyte Common Antigens - analysis

T-Lymphocytes - metabolism

Aged, 80 and over

Adult

Female

Immunoblastic Lymphadenopathy - pathology

Immunophenotyping - methods

Retrospective Studies

T-Lymphocytes - pathology

Immunoblastic Lymphadenopathy - genetics

Neprilysin - analysis

Antigens, CD7 - analysis

Lymphoma, T-Cell - genetics

Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor - genetics

Receptors, Complement 3d - analysis

CD3 Complex - analysis

Bone Marrow - pathology

T-Lymphocytes - chemistry

Lymphoma, T-Cell - pathology

Aged

Immunoblastic Lymphadenopathy - immunology

Details

Title: Subtitle: Flow cytometric features of angioimmunoblastic T-cell lymphoma
Creators: Weina Chen - Department of Pathology, University of Texas Southwestern Medical Center, Dallas, USA
Melissa V Kesler
Nitin J Karandikar
Robert W McKenna
Steven H Kroft
Resource Type: Journal article
Publication Details: Cytometry. Part B, Clinical cytometry, Vol.70(3), pp.142-148
DOI: 10.1002/cyto.b.20107
PMID: 16572417
NLM abbreviation: Cytometry B Clin Cytom
ISSN: 1552-4949
eISSN: 1552-4957
Publisher: United States
Language: English
Date published: 05/2006
Academic Unit: Pathology
Record Identifier: 9984047768802771

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