Flow-mediated dilation attenuates constriction of large coronary arteries to serotonin

Kathryn G Lamping; William P Dole

doi:10.1152/ajpheart.1988.255.6.H1317

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Flow-mediated dilation attenuates constriction of large coronary arteries to serotonin

Journal article

Peer reviewed

Flow-mediated dilation attenuates constriction of large coronary arteries to serotonin

Kathryn G Lamping and William P Dole

The American journal of physiology, Vol.255(6 Pt 2), pp.H1317-H1324

12/1988

DOI: 10.1152/ajpheart.1988.255.6.H1317

PMID: 3144185

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Abstract

We tested the hypothesis that flow-mediated endothelium-dependent dilation can attenuate humorally mediated constriction of large coronary arteries in vivo. Accordingly, we measured constriction of large coronary arteries to serotonin when flow was allowed to increase and when flow was constant in the presence and absence of endothelium. The left anterior descending coronary artery of anesthetized dogs was perfused at constant pressure (100 mmHg) and diameter measured with an ultrasonic dimension gauge. Coronary flow was measured with an in-line electromagnetic flow probe. Flow-mediated dilation was demonstrated by measuring diameter (D) after increased flow in response to adenosine (1 mg/min ic). Adenosine (n = 9) increased flow (341 +/- 69%) and resulted in large artery dilation [diameter change (delta D) = 101 +/- 54 microns], which was abolished by maintaining constant flow (distal snare) or by removing endothelium. Serotonin (n = 9, 50 micrograms/min ic) increased flow (298 +/- 45%) while simultaneously decreasing large artery diameter (delta D = -58 +/- 22 microns). When flow was kept constant, serotonin produced a greater constriction (delta D = -173 +/- 29 microns). After the removal of endothelium (n = 10), constrictor responses to serotonin were similar when flow increased (delta D = -84 +/- 13 microns) and when flow was kept constant (delta D = -79 +/- 23 microns). We conclude that flow-mediated dilation of large coronary arteries can attenuate constriction to serotonin and that this effect is dependent on endothelium.

Coronary Vessels - drug effects

Coronary Vessels - physiology

Male

Reference Values

Adenosine - pharmacology

Serotonin - pharmacology

Nitroglycerin - pharmacology

Vasodilation

Endothelium, Vascular - physiology

Animals

Dogs

Female

Blood Pressure - drug effects

Coronary Circulation - drug effects

In Vitro Techniques

Details

Title: Subtitle: Flow-mediated dilation attenuates constriction of large coronary arteries to serotonin
Creators: Kathryn G Lamping - Department of Internal Medicine, University of Iowa, Iowa City 52242
William P Dole
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.255(6 Pt 2), pp.H1317-H1324
DOI: 10.1152/ajpheart.1988.255.6.H1317
PMID: 3144185
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Grant note: HL-36816 / NHLBI NIH HHS HL-01606 / NHLBI NIH HHS HL-33272 / NHLBI NIH HHS
Language: English
Date published: 12/1988
Academic Unit: Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984094636702771

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