Journal article
Fluorescence quenching of thionine by reduced nicotinamide adenine dinucleotide
Spectrochimica acta. Part A: Molecular spectroscopy, Vol.48(5), pp.647-651
1992
DOI: 10.1016/0584-8539(92)80209-F
Abstract
Reduced nicotinamide adenine dinucleotide (NADH) is shown to quench the fluorescence of thionine. Quenching of thionine is extremely efficient with a half quenching concentration of only 16.1 × 10
−6 M NADH. A Stern—Volmer plot is linear over the NADH concentration range from 1 to 20μM. The corresponding Stern—Volmer quenching constant is 6.2 × 10
4 M
−1 and the limit of detection for NADH measurements is 1.6 × 10
−6 M. Process of quenching is attributed to the formation of an exciplex between thionine and NADH. Potential analytical features of this system are discussed.
Details
- Title: Subtitle
- Fluorescence quenching of thionine by reduced nicotinamide adenine dinucleotide
- Creators
- Ashutosh Sharma - Molecular Biophysics and Optics Division, Biotechnology Centre, Cranfield Institute of Technology, Cranfield, Bedfordshire MK43 0AL, U.KMark A Arnold - Department of Chemistry, University of Iowa, Iowa City, IA 52242, U.S.A
- Resource Type
- Journal article
- Publication Details
- Spectrochimica acta. Part A: Molecular spectroscopy, Vol.48(5), pp.647-651
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0584-8539(92)80209-F
- ISSN
- 0584-8539
- Language
- English
- Date published
- 1992
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Center for Biocatalysis and Bioprocessing; Chemistry
- Record Identifier
- 9984216673902771
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