Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica

Marta Sa; Carlo G. Bertinetto; Narcis Ferrer-Ledo; Jeroen J. Jansen; Rene Wijffels; Joao G. Crespo; Maria Barbosa; Claudia F. Galinha

doi:10.1038/s41598-020-64628-7

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Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica

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Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica

Marta Sa, Carlo G. Bertinetto, Narcis Ferrer-Ledo, Jeroen J. Jansen, Rene Wijffels, Joao G. Crespo, Maria Barbosa and Claudia F. Galinha

Scientific reports, Vol.10(1), pp.7688-7688

05/06/2020

DOI: 10.1038/s41598-020-64628-7

PMCID: PMC7203222

PMID: 32376848

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Abstract

Online monitoring of algal biotechnological processes still requires development to support economic sustainability. In this work, fluorescence spectroscopy coupled with chemometric modelling is studied to monitor simultaneously several compounds of interest, such as chlorophyll and fatty acids, but also the biomass as a whole (cell concentration). Fluorescence excitation-emission matrices (EEM) were acquired in experiments where different environmental growing parameters were tested, namely light regime, temperature and nitrogen (replete or deplete medium). The prediction models developed have a high R-2 for the validation data set for all five parameters monitored, specifically cell concentration (0.66), chlorophyll (0.78), and fatty acid as total (0.78), saturated (0.81) and unsaturated (0.74). Regression coefficient maps of the models show the importance of the pigment region for all outputs studied, and the protein-like fluorescence region for the cell concentration. These results demonstrate for the first time the potential of fluorescence spectroscopy for in vivo and real-time monitoring of these key performance parameters during Nannochloropsis oceanica cultivation.

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Title: Subtitle: Fluorescence spectroscopy and chemometrics for simultaneous monitoring of cell concentration, chlorophyll and fatty acids in Nannochloropsis oceanica
Creators: Marta Sa - Wageningen University & Research
Carlo G. Bertinetto - Radboud Institute for Molecular Life Sciences
Narcis Ferrer-Ledo - Wageningen University & Research
Jeroen J. Jansen - Radboud University Nijmegen
Rene Wijffels - Wageningen University & Research
Joao G. Crespo - Universidade Nova de Lisboa
Maria Barbosa - Wageningen University & Research
Claudia F. Galinha - Universidade Nova de Lisboa
Resource Type: Journal article
Publication Details: Scientific reports, Vol.10(1), pp.7688-7688
DOI: 10.1038/s41598-020-64628-7
PMID: 32376848
PMCID: PMC7203222
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: Springer Nature
Number of pages: 10
Grant note: SFRH/BD/108894/2015 / FCT/MCTES; Fundacao para a Ciencia e a Tecnologia (FCT) SFRH/BD/108894/2015 / Fundao para a Cincia e a Tecnologia UID/QUI/50006/2019 / Associate Laboratory for Green Chemistry - LAQV - FCT/MCTES; Fundacao para a Ciencia e a Tecnologia (FCT) 745754 / project "Microalgae As a Green source from Nutritional Ingredients for Food/Feed and Ingredients for Cosmetics by cost effective New Technologies" [MAGNIFICENT] - Bio-based Industries Joint Technology Initiative under European Commission
Language: English
Date published: 05/06/2020
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984397923502771

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