Folic acid supplementation use and the MTHFR C677T polymorphism in orofacial clefts etiology: An individual participant data pooled-analysis

Azeez Butali; Julian Little; Cécile Chevrier; Sylvian Cordier; Regine Steegers-Theunissen; Astanand Jugessur; Bola Oladugba; Peter A Mossey

doi:10.1002/bdra.23133

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Folic acid supplementation use and the MTHFR C677T polymorphism in orofacial clefts etiology: An individual participant data pooled-analysis

Journal article

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Peer reviewed

Folic acid supplementation use and the MTHFR C677T polymorphism in orofacial clefts etiology: An individual participant data pooled-analysis

Azeez Butali, Julian Little, Cécile Chevrier, Sylvian Cordier, Regine Steegers-Theunissen, Astanand Jugessur, Bola Oladugba and Peter A Mossey

Birth defects research. A Clinical and molecular teratology, Vol.97(8), pp.509-514

08/2013

DOI: 10.1002/bdra.23133

PMCID: PMC3745533

PMID: 23670871

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Abstract

This study examines gene-environment interaction between the MTHFR C667T polymorphism and folic acid in the etiology of orofacial clefts (OFC). We used a pooled-analytical approach on four studies that used similar methods.\nWe used logistic regression to analyze the pooled sample of 1149 isolated cases and 1161 controls. Fetal and maternal MTHFR C677T genotypes, and maternal periconceptional exposure to smoking, alcohol, vitamin containing folic acid and folic acid supplements were contrasted between the cleft types [non-syndromic clefts lip or without cleft palate (CL(P)) and non-syndromic cleft palate (CP)] and control groups.\nThere was a reduced risk of CL(P) with maternal folic acid use (p = 0.008; OR = 0.70, 95% CI: 0.65-0.94) and with supplements containing folic acid (p = 0.028, OR = 0.80, 95% CI: 0.65-0.94). Maternal smoking increased the risk of both CL(P) (p < 10 e-3; OR = 1.62, 95% CI: 1.35-1.95) and CP (p = 0.028; OR = 1.38, 95% CI: 1.04-1.83). No significant risk was observed with either maternal or fetal MTHFR C677T genotypes.\nThis individual participant data (IPD) meta-analysis affords greater statistical power and can help alleviate the problems associated with aggregate-level data-sharing. The result of this IPD meta-analysis is consistent with previous reports suggesting that folic acid and smoking influence OFC outcomes.

Ethanol - metabolism

Humans

Risk Factors

Male

Cleft Palate - genetics

Brain - abnormalities

Case-Control Studies

Smoking - metabolism

Cleft Lip - genetics

Methylenetetrahydrofolate Reductase (NADPH2) - genetics

Cleft Palate - etiology

Folic Acid - metabolism

Maternal Exposure - adverse effects

Adult

Female

Polymorphism, Single Nucleotide

Dietary Supplements

Cleft Lip - etiology

Details

Title: Subtitle: Folic acid supplementation use and the MTHFR C677T polymorphism in orofacial clefts etiology: An individual participant data pooled-analysis
Creators: Azeez Butali - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USA. Azeez-butali@uiowa.edu
Julian Little
Cécile Chevrier
Sylvian Cordier
Regine Steegers-Theunissen
Astanand Jugessur
Bola Oladugba
Peter A Mossey
Resource Type: Journal article
Publication Details: Birth defects research. A Clinical and molecular teratology, Vol.97(8), pp.509-514
DOI: 10.1002/bdra.23133
PMID: 23670871
PMCID: PMC3745533
NLM abbreviation: Birth Defects Res A Clin Mol Teratol
ISSN: 1542-0752
eISSN: 1542-0760
Publisher: Wiley; United States
Grant note: K99-DE022378-01 / NIDCR NIH HHS\nK99 DE022378 / NIDCR NIH HHS\nR00 DE022378 / NIDCR NIH HHS
Language: English
Date published: 08/2013
Academic Unit: Oral Pathology, Radiology and Medicine; Stead Family Department of Pediatrics; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984066090402771

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