Journal article
Force generation and phosphate release steps in skinned rabbit soleus slow-twitch muscle fibers
Biophysical journal, Vol.73(2), pp.878-894
08/1997
DOI: 10.1016/S0006-3495(97)78121-9
PMCID: PMC1180985
PMID: 9251805
Abstract
The force-generation and phosphate-release steps of the cross-bridge cycle in rabbit soleus slow-twitch muscle fibers (STF) were investigated using sinusoidal analysis, and the results were compared with those of rabbit psoas fast-twitch fibers (FTF). Single fiber preparations were activated at pCa 4.40 and ionic strength 180 mM at 20 degrees C. The effects of inorganic phosphate (Pi) concentrations on three exponential processes, B, C, and D, were studied. Results are consistent with the following cross-bridge scheme: [formula: see text] where A is actin, M is myosin, D is MgADP, and P is inorganic phosphate. The values determined are k4 = 5.7 +/- 0.5 s-1 (rate constant of isomerization step, N = 9, mean +/- SE), k-4 = 4.5 +/- 0.5 s-1 (rate constant of reverse isomerization), K4 = 1.37 +/- 0.13 (equilibrium constant of the isomerization), and K5 = 0.18 +/- 0.01 mM-1 (Pi association constant). The isomerization step (k4) in soleus STF is 20 times slower, and its reversal (k-4) is 20 times slower than psoas fibers. Consequently, the equilibrium constant of the isomerization step (K4) is the same in these two types of fibers. The Pi association constant (K5) is slightly higher in STF than in FTF, indicating that Pi binds to cross-bridges slightly more tightly in STF than FTF. By correlating the cross-bridge distribution with isometric tension, it was confirmed that force is generated during the isomerization (step 4) of the AMDP state and before Pi release in soleus STF.
Details
- Title: Subtitle
- Force generation and phosphate release steps in skinned rabbit soleus slow-twitch muscle fibers
- Creators
- Gang Wang - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City 52242, USAMasataka Kawai - Department of Anatomy and Cell Biology, College of Medicine, University of Iowa, Iowa City 52242, USA
- Resource Type
- Journal article
- Publication Details
- Biophysical journal, Vol.73(2), pp.878-894
- DOI
- 10.1016/S0006-3495(97)78121-9
- PMID
- 9251805
- PMCID
- PMC1180985
- ISSN
- 0006-3495
- eISSN
- 1542-0086
- Language
- English
- Date published
- 08/1997
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025404802771
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