Foxj1 is required for apical localization of ezrin in airway epithelial cells

Tao Huang; You You; Melanie S Spoor; Edward J Richer; Vrinda V Kudva; Renee C Paige; Michael P Seiler; Janice M Liebler; Joseph Zabner; Charles G Plopper; Steven L Brody

doi:10.1242/jcs.00830

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Foxj1 is required for apical localization of ezrin in airway epithelial cells

Journal article

Peer reviewed

Foxj1 is required for apical localization of ezrin in airway epithelial cells

Tao Huang, You You, Melanie S Spoor, Edward J Richer, Vrinda V Kudva, Renee C Paige, Michael P Seiler, Janice M Liebler, Joseph Zabner, Charles G Plopper, …

Journal of cell science, Vol.116(Pt 24), pp.4935-4945

12/15/2003

DOI: 10.1242/jcs.00830

PMID: 14625387

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Abstract

Establishment and maintenance of epithelial cell polarity depend on cytoskeletal organization and protein trafficking to polarized cortical membranes. ERM (ezrin, radixin, moesin) family members link polarized proteins with cytoskeletal actin. Although ERMs are often considered to be functionally similar, we found that, in airway epithelial cells, apical localization of ERMs depend on cell differentiation and is independently regulated. Moesin was present in the apical membrane of all undifferentiated epithelial cells. However, in differentiated cells, ezrin and moesin were selectively localized to apical membranes of ciliated airway cells and were absent from secretory cells. To identify regulatory proteins required for selective ERM trafficking, we evaluated airway epithelial cells lacking Foxj1, an F-box factor that directs programs required for cilia formation at the apical membrane. Interestingly, Foxj1 expression was also required for localization of apical ezrin, but not moesin. Additionally, membrane-cytoskeletal and threonine-phosphorylated ezrin were decreased in Foxj1-null cells, consistent with absent apical ezrin. Although apical moesin expression was present in null cells, it could not compensate for ezrin because ERM-associated EBP50 and the beta2 adrenergic receptor failed to localize apically in the absence of Foxj1. These findings indicate that Foxj1 regulates ERM proteins differentially to selectively direct the apical localization of ezrin for the organization of multi-protein complexes in apical membranes of airway epithelial cells.

Cytoskeletal Proteins

Immunohistochemistry

Phosphorylation

Epithelial Cells - metabolism

Cells, Cultured

Sodium-Hydrogen Exchangers

Trachea - metabolism

Phosphoproteins - metabolism

Cilia - metabolism

Receptors, Adrenergic, beta-2 - metabolism

Protein Transport

DNA-Binding Proteins - metabolism

Animals

Carrier Proteins - metabolism

Cytoskeleton - metabolism

Cell Polarity - physiology

Trans-Activators - metabolism

Cell Membrane - metabolism

Mice

Microfilament Proteins - metabolism

Adenoviridae - metabolism

Epithelial Cells - cytology

Cell Differentiation - physiology

Forkhead Transcription Factors

Details

Title: Subtitle: Foxj1 is required for apical localization of ezrin in airway epithelial cells
Creators: Tao Huang - Department of Internal Medicine, Washington University School of Medicine, St Louis, MO 63110, USA. brodys@msnotes.wustl.edu
You You
Melanie S Spoor
Edward J Richer
Vrinda V Kudva
Renee C Paige
Michael P Seiler
Janice M Liebler
Joseph Zabner
Charles G Plopper
Steven L Brody
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.116(Pt 24), pp.4935-4945
DOI: 10.1242/jcs.00830
PMID: 14625387
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Grant note: P30 DK-52574 / NIDDK NIH HHS
Language: English
Date published: 12/15/2003
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984094529402771

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