Journal article
Free solution mobility of DNA molecules containing variable numbers of cationic phosphoramidate internucleoside linkages
Journal of Chromatography A, Vol.883(1), pp.267-275
2000
DOI: 10.1016/S0021-9673(00)00415-5
PMID: 10910219
Abstract
The free solution electrophoretic mobility of an 118-base pair DNA fragment containing zero, three, six or nine cationic phosphoramidate internucleoside linkages has been measured by capillary electrophoresis. The electrophoretic mobility decreases with the increasing number of cationic phosphoramidate linkages, as expected because of the reduced negative charge on the DNA molecules. The decrease in mobility is approximately linear for DNA molecules containing three and six cationic phosphoramidate linkages, but begins to level off when nine cationic phosphoramidate linkages have been added. The mobility also varies somewhat depending on whether the modified phosphoramidate linkages are located at the 5′- or 3′-end of the DNA molecule.
Details
- Title: Subtitle
- Free solution mobility of DNA molecules containing variable numbers of cationic phosphoramidate internucleoside linkages
- Creators
- Nancy C Stellwagen - Department of Biochemistry, University of Iowa, Iowa City, IA 52242, USASoffia Magnusdottir - Department of Agricultural and Industrial Biotechnologies, University of Verona, Strada Le Grazie, Ca’ Vignal, 37134 Verona, ItalyJohn M Dagle - Department of Pediatrics, University of Iowa, Iowa City, IA 52242, USACecilia Gelfi - ITBA, CNR, Via Fratelli Cervi No. 93, Segrate 20090, Milan, ItalyPier Giorgio Righetti - Department of Agricultural and Industrial Biotechnologies, University of Verona, Strada Le Grazie, Ca’ Vignal, 37134 Verona, Italy
- Resource Type
- Journal article
- Publication Details
- Journal of Chromatography A, Vol.883(1), pp.267-275
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0021-9673(00)00415-5
- PMID
- 10910219
- ISSN
- 0021-9673
- Language
- English
- Date published
- 2000
- Academic Unit
- Stead Family Department of Pediatrics; Epidemiology; Biochemistry and Molecular Biology; Neonatology
- Record Identifier
- 9984025397002771
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