Journal article
Free solution mobility of small single-stranded oligonucleotides with variable charge densities
Electrophoresis, Vol.24(19-20), pp.3323-3329
10/2003
DOI: 10.1002/elps.200305589
PMID: 14595678
Abstract
The free solution mobilities of six single-stranded 16-nucleotide DNA oligomers with the same sequence, containing up to 11 neutral phosphoramidate internucleoside linkages, have been measured by capillary electrophoresis. The mobilities of the partially charged oligomers increase linearly with the logarithm of increasing charge density, as predicted by the Manning theory of electrophoresis (G. S. Manning, J. Phys. Chem. 1981, 85, 1506-1515). For comparison, the mobilities of eight fully charged single-stranded oligomers containing similar numbers of charged phosphate residues have also been measured. The mobilities of the variable length, fully charged oligomers increase more rapidly with the increasing number of charged phosphate residues than the mobilities of the constant size, partially charged phosphoramidate derivatives, because of the larger diffusion coefficients of the modified oligomers.
Details
- Title: Subtitle
- Free solution mobility of small single-stranded oligonucleotides with variable charge densities
- Creators
- Qian Dong - Department of Biochemistry, University of Iowa, Iowa City 52242, USAEarle StellwagenJohn M DagleNancy C Stellwagen
- Resource Type
- Journal article
- Publication Details
- Electrophoresis, Vol.24(19-20), pp.3323-3329
- DOI
- 10.1002/elps.200305589
- PMID
- 14595678
- NLM abbreviation
- Electrophoresis
- ISSN
- 0173-0835
- eISSN
- 1522-2683
- Publisher
- Germany
- Grant note
- GM61009 / NIGMS NIH HHS
- Language
- English
- Date published
- 10/2003
- Academic Unit
- Stead Family Department of Pediatrics; Epidemiology; Biochemistry and Molecular Biology; Neonatology
- Record Identifier
- 9984024547202771
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