Frequency-Dependent Kinetics and Prevalence of Kiss-and-Run and Reuse at Hippocampal Synapses Studied with Novel Quenching Methods

Nobutoshi C Harata; Sukwoo Choi; Jason L Pyle; Alexander M Aravanis; Richard W Tsien

doi:10.1016/j.neuron.2005.12.018

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Frequency-Dependent Kinetics and Prevalence of Kiss-and-Run and Reuse at Hippocampal Synapses Studied with Novel Quenching Methods

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Frequency-Dependent Kinetics and Prevalence of Kiss-and-Run and Reuse at Hippocampal Synapses Studied with Novel Quenching Methods

Nobutoshi C Harata, Sukwoo Choi, Jason L Pyle, Alexander M Aravanis and Richard W Tsien

Neuron (Cambridge, Mass.), Vol.49(2), pp.243-256

2006

DOI: 10.1016/j.neuron.2005.12.018

PMID: 16423698

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Abstract

The kinetics of exo-endocytotic recycling could restrict information transfer at central synapses if neurotransmission were entirely reliant on classical full-collapse fusion. Nonclassical fusion retrieval by kiss-and-run would be kinetically advantageous but remains controversial. We used a hydrophilic quencher, bromophenol blue (BPB), to help detect nonclassical events. Upon stimulation, extracellular BPB entered synaptic vesicles and quenched FM1-43 fluorescence, indicating retention of FM dye beyond first fusion. BPB also quenched fluorescence of VAMP (synaptobrevin-2)-EGFP, thus indicating the timing of first fusion of vesicles in the total recycling pool. Comparison with FM dye destaining revealed that kiss-and-run strongly prevailed over full-collapse fusion at low frequency, giving way to a near-even balance at high frequency. Quickening of kiss-and-run vesicle reuse was also observed at higher frequency in the average single vesicle fluorescence response. Kiss-and-run and reuse could enable hippocampal nerve terminals to conserve scarce vesicular resources when responding to widely varying input patterns.

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Title: Subtitle: Frequency-Dependent Kinetics and Prevalence of Kiss-and-Run and Reuse at Hippocampal Synapses Studied with Novel Quenching Methods
Creators: Nobutoshi C Harata - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305
Sukwoo Choi - Seoul National University, School of Biological Sciences, Kwanak Gu, Sillim 9 Dong San 56-11, Seoul 151-742, South Korea
Jason L Pyle - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305
Alexander M Aravanis - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305
Richard W Tsien - Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, California 94305
Resource Type: Journal article
Publication Details: Neuron (Cambridge, Mass.), Vol.49(2), pp.243-256
DOI: 10.1016/j.neuron.2005.12.018
PMID: 16423698
NLM abbreviation: Neuron
ISSN: 0896-6273
eISSN: 1097-4199
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000025, name: National Institute of Mental Health, award: MH64070; DOI: 10.13039/501100003625, name: Ministry of Health and Welfare, award: 02-PJ1-PG1-CH06-0001
Language: English
Date published: 2006
Academic Unit: Molecular Physiology and Biophysics
Record Identifier: 9984025576602771

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