Frequency of Isolation of Porphyromonas Species from Infected Dog and Cat Bite Wounds in Humans and Their Characterization by Biochemical Tests and Arbitrarily Primed-Polymerase Chain Reaction Fingerprinting

D. M. Citron; S. Hunt Gerardo; M. C. Claros; F. Abrahamian; D. Talan; E. J. C. Goldstein

doi:10.1093/clinids/23.Supplement_1.S78

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Frequency of Isolation of Porphyromonas Species from Infected Dog and Cat Bite Wounds in Humans and Their Characterization by Biochemical Tests and Arbitrarily Primed-Polymerase Chain Reaction Fingerprinting

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Frequency of Isolation of Porphyromonas Species from Infected Dog and Cat Bite Wounds in Humans and Their Characterization by Biochemical Tests and Arbitrarily Primed-Polymerase Chain Reaction Fingerprinting

D. M. Citron, S. Hunt Gerardo, M. C. Claros, F. Abrahamian, D. Talan and E. J. C. Goldstein

Clinical infectious diseases, Vol.23(Supplement-1), pp.S78-S82

12/1996

DOI: 10.1093/clinids/23.Supplement_1.S78

PMID: 8953111

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Abstract

We isolated 40 strains of Porphyromonas (formerly Bacteroides) species from 29 of 102 cat and dog bite wounds in humans. P. salivosa, P. gingivalis, and P. canoris were the most frequent isolates. A comparison of the RapID ANA II system (Innovative Diagnostic Systems, Norcross, GA), An-IDENT panels (bioMérieux, st. Louis), and API ZYM strips (bioMérieux) showed that the latter kit best characterized these isolates because it included tests for trypsin and chymotrypsin activity; however, the tests for glycosidase activity in this kit were less sensitive than were those in the other kits. None of the biochemical systems was able to identify all species. Arbitrarily primed-polymerase chain reaction fingerprinting with a nonspecific single primer, T3B, yielded distinct profiles for type strains and for the clinical isolates, suggesting that some of the isolates represented previously undescribed species. Growth of these species took ≥5 days; therefore, laboratories should incubate anaerobic plates from bite wound cultures for ≥7 days to assure isolation of these common pathogens.

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Title: Subtitle: Frequency of Isolation of Porphyromonas Species from Infected Dog and Cat Bite Wounds in Humans and Their Characterization by Biochemical Tests and Arbitrarily Primed-Polymerase Chain Reaction Fingerprinting
Creators: D. M. Citron - UCLA Medical Center
S. Hunt Gerardo - R. M. Alden Research Laboratory, Santa Monica-UCLA Medical Center, Santa Monica, Sylmar, Los Angeles, California, USA, Leipzig, Germany
M. C. Claros - UCLA Medical Center
F. Abrahamian - UCLA Medical Center
D. Talan - UCLA Medical Center
E. J. C. Goldstein - R. M. Alden Research Laboratory, Santa Monica-UCLA Medical Center, Santa Monica, Sylmar, Los Angeles, California, USA, Leipzig, Germany
Resource Type: Journal article
Publication Details: Clinical infectious diseases, Vol.23(Supplement-1), pp.S78-S82
Publisher: The University of Chicago Press
DOI: 10.1093/clinids/23.Supplement_1.S78
PMID: 8953111
ISSN: 1058-4838
eISSN: 1537-6591
Language: English
Date published: 12/1996
Academic Unit: Emergency Medicine; Internal Medicine
Record Identifier: 9984297146702771

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