Frequency response of pig intervertebral disc cells subjected to dynamic hydrostatic pressure

Mehran Kasra; W David Merryman; Kristen N Loveless; Vijay K Goel; James D Martin; Joseph A Buckwalter

doi:10.1002/jor.20253

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Frequency response of pig intervertebral disc cells subjected to dynamic hydrostatic pressure

Journal article

Open access

Peer reviewed

Frequency response of pig intervertebral disc cells subjected to dynamic hydrostatic pressure

Mehran Kasra, W David Merryman, Kristen N Loveless, Vijay K Goel, James D Martin and Joseph A Buckwalter

Journal of orthopaedic research, Vol.24(10), pp.1967-1973

10/2006

DOI: 10.1002/jor.20253

PMID: 16900539

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Abstract

The pathogenesis of vibration-induced disorders of intervertebral disc at the cellular level is largely unknown. Dynamic loads with frequencies close to that of the in vivo human spine resonant frequency (4-6 Hz) have a destructive effect, which may induce extracellular disc matrix (ECM) degradation. To investigate this issue, three-dimensional (3D) alginate cultures of normal pig intervertebral disc nucleus and inner annulus cells were tested under dynamic hydrostatic loading. Alginate cultures of each region were divided into six groups; five groups were exposed to cyclic hydrostatic pressures of frequencies 1, 3, 5, 8, and 10 Hz with the same amplitude (1 MPa), and group 6 was the control group (no loading). Cultures of different groups were loaded for 3 days (30 min daily) in a hydraulic chamber. Effects of loading frequency on disc collagen and protein metabolism were investigated by measuring 3H-proline-labeled proteins associated with the cells in the extracellular matrix and release of 3H-proline-labeled molecules into culture medium. The results indicated a poor synthesis rate and more degradation near the 5 Hz frequency. The repeatability of experiments was verified by performing two experiments with the same protocol. Both experiments indicated that a threshold frequency of around 5 Hz disrupted protein metabolism.

Hydrostatic Pressure - adverse effects

Extracellular Matrix Proteins - secretion

Intervertebral Disc - cytology

Spinal Diseases - etiology

Animals

Vibration - adverse effects

Intervertebral Disc - secretion

Swine

Cells, Cultured

Cell Culture Techniques

Details

Title: Subtitle: Frequency response of pig intervertebral disc cells subjected to dynamic hydrostatic pressure
Creators: Mehran Kasra - Department of Mechanical Engineering, McMaster University, Hamilton, Ontario, Canada L8S 4L7, and Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and Clinics, Iowa City 52242, USA. kasram@mcmaster.ca
W David Merryman
Kristen N Loveless
Vijay K Goel
James D Martin
Joseph A Buckwalter
Resource Type: Journal article
Publication Details: Journal of orthopaedic research, Vol.24(10), pp.1967-1973
DOI: 10.1002/jor.20253
PMID: 16900539
NLM abbreviation: J Orthop Res
ISSN: 0736-0266
eISSN: 1554-527X
Publisher: United States
Language: English
Date published: 10/2006
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
Record Identifier: 9984040544602771

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