Journal article
Frequent breakpoints in the 3p14.2 fragile site, FRA3B, in pancreatic tumors
Cancer research (Chicago, Ill.), Vol.56(19), pp.4347-4350
10/01/1996
PMID: 8813121
Abstract
FRA3B, at chromosomal band 3p14.2, is the most active common fragile site in the human genome. Homozygous deletions in the region of FRA3B have been observed in many types of solid tumors. Recently, the FHIT gene was reported to span FRA3B and was shown to be homozygously deleted in several gastric and colonic tumor cell lines. Several microsatellite markers that precisely define the 1.0-Mb region surrounding FRA3B and FHIT have been utilized, along with other 3p microsatellites, to analyze the loss of 3p sequences in 25 primary pancreatic adenocarcinomas. The high density of microsatellite markers in the 3pl4.2 region enabled us to both identify losses within and flanking FRA3B in pancreatic cancer and define the breakpoints. We observed loss of het erozygosity of 3pl4.2 markers in 16 of 25 pancreatic tumors and loss of heterozygosity of 3p markers outside of 3pl4.2 in only 2 of 25 tumors of this type. There appears to be a dramatic clustering of chromosomal breakpoints at 3pl4.2 in and immediately distal to FRA3B in pancreatic cancer. We detected no homozygous deletions in this region.
Details
- Title: Subtitle
- Frequent breakpoints in the 3p14.2 fragile site, FRA3B, in pancreatic tumors
- Creators
- Ravi Shridhar - Wayne State UniversityViji Shridhar - Wayne State UniversityXiaohong Wang - Wayne State UniversityWilliam Paradee - Wayne State UniversityMichael Dugan - Wayne State UniversityFazul Sarkar - Wayne State UniversityCharles Wilke - University of Michigan Medical SchoolThomas W. Glover - University of Michigan–Ann ArborVainutis K Vaitkevicius - Wayne State UniversityDavid I. Smith - Wayne State University
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.56(19), pp.4347-4350
- PMID
- 8813121
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Grant note
- CA48031 / NCI NIH HHS CA43222 / NCI NIH HHS
- Language
- English
- Date published
- 10/01/1996
- Academic Unit
- Medicine Administration
- Record Identifier
- 9984622753002771
Metrics
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