Journal article
Frequent breakpoints in the region surrounding FRA3B in sporadic renal cell carcinomas
Oncogene, Vol.14(11), pp.1269-1277
03/20/1997
DOI: 10.1038/sj.onc.1201100
PMID: 9178887
Abstract
The constitutive fragile site at chromosomal band 3p14.2, FRA3B, is the most active common fragile site in the human genome. We have localized aphidicolin-induced breakpoints to two distinct clusters, separated by 200 Kb, in FRA3B (Paradee et al., 1996). Sequence analysis of these regions identified two polymorphic microsatellite markers immediately adjacent to each of these breakpoint clusters. In this report we have used these two new microsatellites and 14 additional 3p microsatellites to analyse chromosome 3p breakage and loss in 94 sporadic RCC samples, including nonpapillary, papillary and oncocytomas. We have found heterozygous loss of 3p14 sequences in >60% of the RCC samples, including both clear cell and papillary renal cell carcinomas. We have found frequent breakage in the region immediately surrounding FRA3B, demonstrating that FRA3B does play a role in chromosome breakage and loss in RCC. In contrast to other reports, >50% of the papillary tumors also showed LOH of 3p markers. We also observed microsatellite instability (MIN) with most of the tested markers in seven of eight oncocytomas and one of 69 clear cell carcinomas. The MIN in some oncocytomas was of the RER+ (replication error) type I phenotype. None of the five 3p14.2 markers detected any homozygous deletions in tumor samples, but 69/94 (73%) of the tumors had LOH for the region, which includes the recently identified FHIT gene.
Details
- Title: Subtitle
- Frequent breakpoints in the region surrounding FRA3B in sporadic renal cell carcinomas
- Creators
- Viji ShridharLiang Wang - The Barbara Ann Karmanos Cancer InstituteRita Rosati - The Barbara Ann Karmanos Cancer InstituteWilliam Paradee - Emory UniversityRavi ShridharChadwick Mullins - Wayne State UniversityWael SakrDavid Grignon - Wayne State UniversityOrlando Miller - Wayne State UniversityQi Sun - Emory UniversityJohn Petros - Emory UniversityDavid Smith
- Resource Type
- Journal article
- Publication Details
- Oncogene, Vol.14(11), pp.1269-1277
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/sj.onc.1201100
- PMID
- 9178887
- ISSN
- 0950-9232
- eISSN
- 1476-5594
- Language
- English
- Date published
- 03/20/1997
- Academic Unit
- Medicine Administration
- Record Identifier
- 9984622753102771
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