Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina

Christie K Campla; Ulisse Bocchero; Ryan Strickland; Jacob Nellissery; Jayshree Advani; Irina Ignatova; Dhiraj Srivastava; Angel M Aponte; Yuchen Wang; Jessica Gumerson; Kirill Martemyanov; Nikolai O Artemyev; Johan Pahlberg; Anand Swaroop

doi:10.1523/ENEURO.0348-22.2022

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Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina

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Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina

Christie K Campla, Ulisse Bocchero, Ryan Strickland, Jacob Nellissery, Jayshree Advani, Irina Ignatova, Dhiraj Srivastava, Angel M Aponte, Yuchen Wang, Jessica Gumerson, …

ENeuro, Vol.9(5), ENEURO.0348-22.2022

09/30/2022

DOI: 10.1523/ENEURO.0348-22.2022

PMCID: PMC9581579

PMID: 36180221

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Abstract

Trafficking of transducin (Gat) in rod photoreceptors is critical for adaptive and modulatory responses of the retina to varying light intensities. In addition to fine-tuning phototransduction gain in rod outer segments (OSs), light-induced translocation of Gat to the rod synapse enhances rod to rod bipolar synaptic transmission. Here, we show that the rod-specific loss of Frmpd1 (FERM and PDZ domain containing 1), in the retina of both female and male mice, results in delayed return of Gat from the synapse back to outer segments in the dark, compromising the capacity of rods to recover from light adaptation. Frmpd1 directly interacts with Gpsm2 (G-protein signaling modulator 2), and the two proteins are required for appropriate sensitization of rod-rod bipolar signaling under saturating light conditions. These studies provide insight into how the trafficking and function of Gat is modulated to optimize the photoresponse and synaptic transmission of rod photoreceptors in a light-dependent manner.

Details

Title: Subtitle: Frmpd1 facilitates trafficking of G-protein transducin and modulates synaptic function in rod photoreceptors of mammalian retina
Creators: Christie K Campla - National Eye Institute
Ulisse Bocchero - National Eye Institute
Ryan Strickland - National Eye Institute
Jacob Nellissery - National Eye Institute
Jayshree Advani - National Eye Institute
Irina Ignatova - Photoreceptor Physiology Group, NEI, NIH, Bethesda, MD 20892, USA.
Dhiraj Srivastava - Roy J. and Lucille A. Carver College of Medicine
Angel M Aponte - National Heart Lung and Blood Institute
Yuchen Wang - Scripps Research Institute
Jessica Gumerson - National Eye Institute
Kirill Martemyanov - Scripps Research Institute
Nikolai O Artemyev - Roy J. and Lucille A. Carver College of Medicine
Johan Pahlberg - National Institutes of Health
Anand Swaroop - National Eye Institute
Resource Type: Journal article
Publication Details: ENeuro, Vol.9(5), ENEURO.0348-22.2022
DOI: 10.1523/ENEURO.0348-22.2022
PMID: 36180221
PMCID: PMC9581579
NLM abbreviation: eNeuro
ISSN: 2373-2822
eISSN: 2373-2822
Grant note: DOI: 10.13039/100000053, name: HHS | NIH | National Eye Institute, award: ZIAEY000450, ZIAEY000546, R01EY12682, R01EY10843, R01EY018139, R01EY028033, K99EY030554; DOI: 10.13039/100000072, name: HHS | NIH | National Institute of Dental and Craniofacial Research; DOI: 10.13039/100000065, name: HHS | NIH | National Institute of Neurological Disorders and Stroke; name: Oskar Huttunen Foundation
Language: English
Date published: 09/30/2022
Academic Unit: Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9984302216102771

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