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Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea
Journal article   Open access   Peer reviewed

Fucoxanthin and docosahexaenoic acid production by cold-adapted Tisochrysis lutea

Fengzheng Gao, Iago Teles Dominguez Cabanelas, Rene H. Wijffels and Maria J. Barbosa
New biotechnology, Vol.66, pp.16-24
01/25/2022
DOI: 10.1016/j.nbt.2021.08.005
PMID: 34500104
url
https://doi.org/10.1016/j.nbt.2021.08.005View
Published (Version of record) Open Access

Abstract

Tisochrysis lutea is an important microalgal species for fucoxanthin and docosahexaenoic acid (DHA) production with an optimum cultivation temperature of approximately 30 degrees C. The aim of the present work was to develop a winter strain with high productivity at 15 degrees C. The response of the original strain to a decrease in temperature from 30 degrees C to 15 degrees C was investigated in continuous turbidostat experiments. This was followed by adaptation for >180 days at 15 degrees C and 2 rounds of sorting for cells with high chlorophyll fluorescence (top 5%) using fluorescence-activated cell sorting (FACS). For the original strain the productivity of biomass, fucoxanthin, and DHA decreased by 92 %, 98 % and 85 % respectively when decreasing the temperature from 30 degrees C to 15 degrees C. In the sorted cold-adapted 'winter strain', biomass, fucoxanthin, and DHA productivities were similar to those at 30 degrees C. In addition, the fucoxanthin concentration increased from 1.11 to 4.24 mg g(-1) dry weight and the polar lipid fraction in total fatty acids increased from 21 % to 55 %. The winter strain showed a robust and stable phenotype after one year of cultivation, expanding the outdoor fucoxanthin and lipid production seasons for this species.
 Biochemical Research Methods Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Life Sciences & Biomedicine Science & Technology

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