Journal article
Full-Length GB Virus C (Hepatitis G Virus) RNA Transcripts Are Infectious in Primary CD4-Positive T Cells
Journal of virology, Vol.74(19), pp.9125-9133
10/2000
DOI: 10.1128/JVI.74.19.9125-9133.2000
PMCID: PMC102111
PMID: 10982359
Abstract
GB virus C (GBV-C or hepatitis G virus) is a recently described flavivirus which frequently leads to chronic viremia in humans. Although GBV-C is associated with acute posttransfusion hepatitis, it is not clear if the virus is pathogenic for humans. We constructed a full-length cDNA from the plasma of a person with chronic GBV-C viremia. Peripheral blood mononuclear cells (PBMCs) transfected with full-length RNA transcripts from this GBV-C clone resulted in viral replication. This was demonstrated by serial passage of virus from cell culture supernatants, detection of increasing concentrations of positive- and negative-sense GBV-C RNA over time, and the detection of the GBV-C E2 antigen by confocal microscopy. In addition, two types of GBV-C particles were identified in cell lysates; these particles had buoyant densities of 1.06 and 1.12 to 1.17 g/ml in sucrose gradients. PBMCs sorted for expression of CD4 contained 100-fold-more GBV-C RNA than CD4-negative cells. Taken together, these data demonstrate that RNA transcripts from GBV-C full-length cDNA are infectious in primary CD4-positive T cells. In contrast, RNA transcripts from an infectious hepatitis C virus clone did not replicate in the same cell culture system. Infectious RNA transcripts from GBV-C cDNA should prove useful for studying viral replication and may allow identification of differences between GBV-C and hepatitis C virus cultivation in vitro.
Details
- Title: Subtitle
- Full-Length GB Virus C (Hepatitis G Virus) RNA Transcripts Are Infectious in Primary CD4-Positive T Cells
- Creators
- Jinhua Xiang - Departments of Internal Medicine and ResearchSabina Wünschmann - Departments of Internal Medicine and ResearchWarren Schmidt - Departments of Internal Medicine and ResearchJianqiang Shao - Departments of Internal Medicine and ResearchJack T Stapleton - Departments of Internal Medicine and Research
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.74(19), pp.9125-9133
- DOI
- 10.1128/JVI.74.19.9125-9133.2000
- PMID
- 10982359
- PMCID
- PMC102111
- NLM abbreviation
- J Virol
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 10/2000
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094669702771
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