Journal article
Full-length genomic analysis of hepatitis B virus isolates in a patient progressing from hepatitis to hepatocellular carcinoma
Journal of medical virology, Vol.64(4), pp.299-304
08/2001
DOI: 10.1002/jmv.1050
PMID: 11468748
Abstract
In hepatitis B virus (HBV)-endemic countries, the majority of hepatocellular carcinoma (HCC) arises in HBV carriers. High frequency of mutations at nucleotides 1762(A-->T) and 1764(G-->A) in the core promoter region have been described in HCC. Due to the differences in genetic backgrounds, environmental risk factors and random cellular insertion sites, it is difficult to analyze the possible roles of HBV variants detected in different HCC patients. In a follow-up cohort study, an HBsAg-positive asymptomatic carrier was diagnosed HCC within 4 years. Eleven full-length HBV isolates, three from the first serum sample obtained 4 years pre-HCC, and eight from serum sample, peri-tumor and tumor tissue post-HCC of this individual were sequenced and used to transfect HepG2 cells. When sequences were compared between pre- and post-HCC isolates, no single mutation common to all post-HCC isolates that differed from pre-HCC isolates was found. Among all 11 isolates, there were 20 predicted amino acid substitutions shared by two or more post-HCC isolates. These were located in the S(5), X(4), core(4), polymerase(4), pre-S1(2) and pre-S2(1) proteins. Possible roles of amino acid substitutions and enhanced replication efficiency in cells transfected by post-HCC isolates are discussed.
Details
- Title: Subtitle
- Full-length genomic analysis of hepatitis B virus isolates in a patient progressing from hepatitis to hepatocellular carcinoma
- Creators
- Xu Lin - Department of Molecular Virology, Shanghai Medical University, Shanghai, ChinaG S QianPei‐Xing LuL WuY M Wen
- Resource Type
- Journal article
- Publication Details
- Journal of medical virology, Vol.64(4), pp.299-304
- Publisher
- United States
- DOI
- 10.1002/jmv.1050
- PMID
- 11468748
- ISSN
- 0146-6615
- eISSN
- 1096-9071
- Language
- English
- Date published
- 08/2001
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001126402771
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