Journal article
Function of bidirectional sensitivity in the otolith organs established by transcription factor Emx2
Nature communications, Vol.13(1), pp.6330-6330
10/24/2022
DOI: 10.1038/s41467-022-33819-3
Abstract
Otolith organs of the inner ear are innervated by two parallel afferent projections to the brainstem and cerebellum. These innervations were proposed to segregate across the line of polarity reversal (LPR) within each otolith organ, which divides the organ into two regions of hair cells (HC) with opposite stereociliary orientation. The relationship and functional significance of these anatomical features are not known. Here, we show regional expression of Emx2 in otolith organs, which establishes LPR, mediates the neuronal segregation across LPR and constitutes the bidirectional sensitivity function. Conditional knockout (cKO) of
Emx2
in HCs lacks LPR.
Tmie
cKO, in which mechanotransduction was abolished selectively in HCs within the Emx2 expression domain also lacks bidirectional sensitivity. Analyses of both mutants indicate that LPR is specifically required for mice to swim comfortably and to traverse a balance beam efficiently, but LPR is not required for mice to stay on a rotating rod.
The inner ear is highly organized, with distinct domains being located across the Line of Polarity Reversal (LPR). Here they show that Emx2 establishes the LPR and bidirectional selectivity of otolith organs, and that loss of the LPR in mice affects swimming and balance.
Details
- Title: Subtitle
- Function of bidirectional sensitivity in the otolith organs established by transcription factor Emx2
- Creators
- Young Rae Ji - National Institutes of HealthYosuke Tona - Bethesda, MD 20892 USA 54 Shogoin-kawahara-cho, Sakyo-ku, Kyoto City, Kyoto, 606-8507 JapanTalah Wafa - Bethesda, MD 20892 USAMatthew E. Christman - Baltimore, MD 21205 USAEdward D. Tourney - Baltimore, MD 21205 USATao Jiang - Bethesda, MD 20892 USA Shanghai, 200031 ChinaSho Ohta - Bethesda, MD 20892 USAHui Cheng - Bethesda, MD 20892 USATracy Fitzgerald - Bethesda, MD 20892 USABernd Fritzsch - Iowa City, IA 52242 USASherri M. Jones - Lincoln, NE 68583 USAKathleen E. Cullen - Baltimore, MD 21205 USADoris K. Wu - Bethesda, MD 20892 USA
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.13(1), pp.6330-6330
- DOI
- 10.1038/s41467-022-33819-3
- eISSN
- 2041-1723
- Publisher
- Nature Publishing Group UK
- Grant note
- ; R01DC018304; RDC002390; R01DC018061 / ; 1ZIADC000021 / ;
- Language
- English
- Date published
- 10/24/2022
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984307252602771
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