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Function of the tetraspanin CD151-α6β1 integrin complex during cellular morphogenesis
Journal article   Open access   Peer reviewed

Function of the tetraspanin CD151-α6β1 integrin complex during cellular morphogenesis

Xin A Zhang, Alexander R Kazarov, Xiuwei Yang, Alexa L Bontrager, Christopher S Stipp and Martin E Hemler
Molecular Biology of the Cell, Vol.13(1), pp.1-11
2002
DOI: 10.1091/mbc.01-10-0481
PMCID: PMC65068
PMID: 11809818
url
https://doi.org/10.1091/mbc.01-10-0481View
Published (Version of record) Open Access

Abstract

Upon plating on basement membrane Matrigel, NIH3T3 cells formed an anastomosing network of cord-like structures, inhibitable by anti-alpha6beta1 integrin antibodies. For NIH3T3 cells transfected with human CD151 protein, the formation of a cord-like network was also inhibitable by anti-CD151 antibodies. Furthermore, CD151 and alpha6beta1 were physically associated within NIH3T3 cells. On removal of the short 8-amino acid C-terminal CD151 tail (by deletion or exchange), exogenous CD151 exerted a dominant negative effect, as it almost completely suppressed alpha6beta1-dependent cell network formation and NIH3T3 cell spreading on laminin-1 (an alpha6beta1 ligand). Importantly, mutant CD151 retained alpha6beta1 association and did not alter alpha6beta1-mediated cell adhesion to Matrigel. In conclusion, the CD151-alpha6beta1 integrin complex acts as a functional unit that markedly influences cellular morphogenesis, with the CD151 tail being of particular importance in determining the "outside-in" functions of alpha6beta1-integrin that follow ligand engagement. Also, antibodies to alpha6beta1 and CD151 inhibited formation of endothelial cell cord-like networks, thus pointing to possible relevance of CD151-alpha6beta1 complexes during angiogenesis.

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