Journal article
Functional Interactions Between the Thrombin Receptor and the T-Cell Antigen Receptor in Human T-Cell Lines
Blood, Vol.90(5), pp.1893-1901
09/01/1997
DOI: 10.1182/blood.V90.5.1893
PMID: 9292522
Abstract
Abstract The proteolytically activated thrombin receptor (TR) is expressed by T lymphocytes, which suggests that thrombin may modulate T-cell activation at sites of hemostatic stress. We examined the relationship between TR function and T-cell activation in the Jurkat human T-cell line and in T-cell lines with defined defects in T-cell antigen receptor (TCR) function. Stimulation with thrombin or the synthetic TR peptide SFLLRN produced intracellular Ca2+ transients in Jurkat cells. As the concentration of TR agonist was increased, peak Ca2+ mobilization increased, but influx of extracellular Ca2+ decreased. TR signaling was enhanced in a TCR-negative Jurkat line and in T-cell lines deficient in the tyrosine kinase lck or the tyrosine phosphatase CD45, both of which are essential for normal TCR function. TCR cross-linking with anti-CD3 IgM desensitized TR signaling in Jurkat cells, but not in CD45-deficient cells. A proteinase-activated receptor (PAR-2)–specific agonist peptide, SLIGKV, produced small Ca2+ transients in both MEG-01 human megakaryocytic cells and Jurkat cells, but was less potent than the TR-specific agonist TFRIFD in both cell types. Like TR signaling, PAR-2 signaling was enhanced in TCR-negative or lck-deficient Jurkat clones. These findings provide evidence for functional cross-talk between proteolytically activated receptors and the TCR.
Details
- Title: Subtitle
- Functional Interactions Between the Thrombin Receptor and the T-Cell Antigen Receptor in Human T-Cell Lines
- Creators
- David E Joyce - From the Veterans Affairs Medical Center, Iowa City, IA; and the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IASteven R Lentz - From the Veterans Affairs Medical Center, Iowa City, IA; and the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IAYan Chen - From the Veterans Affairs Medical Center, Iowa City, IA; and the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IARochelle A Erger - From the Veterans Affairs Medical Center, Iowa City, IA; and the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IAGary A Koretzky - From the Veterans Affairs Medical Center, Iowa City, IA; and the Departments of Internal Medicine and Physiology and Biophysics, University of Iowa College of Medicine, Iowa City, IA
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.90(5), pp.1893-1901
- DOI
- 10.1182/blood.V90.5.1893
- PMID
- 9292522
- NLM abbreviation
- Blood
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 09/01/1997
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094717802771
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