Journal article
Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia
Biomedicines, Vol.9(2), p.100
01/21/2021
DOI: 10.3390/biomedicines9020100
PMID: 33494192
Abstract
Congenital eye defects represent a large class of disorders affecting roughly 21 million children worldwide. Microphthalmia and anophthalmia are relatively common congenital defects, with approximately 20% of human cases caused by mutations in
SOX2.
Recently, we identified the RNA-binding motif protein 24a (Rbm24a) which binds to and regulates
sox2
in zebrafish and mice. Here we show that morpholino knockdown of
rbm24a
leads to microphthalmia and visual impairment. By utilizing sequential injections, we demonstrate that addition of exogenous
sox2
RNA to
rbm24a
-deplete embryos is sufficient to suppress morphological and visual defects. This research demonstrates a critical role for understanding the post-transcriptional regulation of genes needed for development.
Details
- Title: Subtitle
- Functional Role of the RNA-Binding Protein Rbm24a and Its Target sox2 in Microphthalmia
- Creators
- Lindy K Brastrom - Department of Biology, University of Iowa, Iowa City, IA 52245, USAC. Anthony Scott - Mercury Data Science, Houston, TX 77098, USAKai Wang - Department of Biostatistics, University of Iowa, Iowa City, IA 52245, USADiane C Slusarski - Department of Biology, University of Iowa, Iowa City, IA 52245, USA
- Resource Type
- Journal article
- Publication Details
- Biomedicines, Vol.9(2), p.100
- DOI
- 10.3390/biomedicines9020100
- PMID
- 33494192
- NLM abbreviation
- Biomedicines
- ISSN
- 2227-9059
- eISSN
- 2227-9059
- Publisher
- MDPI
- Grant note
- DOI: 10.13039/100000968, name: American Heart Association, award: 17GRNT33670684
- Language
- English
- Date published
- 01/21/2021
- Academic Unit
- Iowa Neuroscience Institute; Biostatistics; Biology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984070570502771
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