Functional and structural changes in a canine model of hereditary primary angle-closure glaucoma

Sinisa D Grozdanic; Helga Kecova; Matthew M Harper; Wijitha Nilaweera; Markus H Kuehn; Randy H Kardon

doi:10.1167/iovs.09-4081

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Functional and structural changes in a canine model of hereditary primary angle-closure glaucoma

Journal article

Open access

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Functional and structural changes in a canine model of hereditary primary angle-closure glaucoma

Sinisa D Grozdanic, Helga Kecova, Matthew M Harper, Wijitha Nilaweera, Markus H Kuehn and Randy H Kardon

Investigative ophthalmology & visual science, Vol.51(1), pp.255-263

01/2010

DOI: 10.1167/iovs.09-4081

PMCID: PMC3258664

PMID: 19661222

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Abstract

To characterize functional and structural changes in a canine model of hereditary primary angle-closure glaucoma. Intraocular pressure (IOP) was evaluated with tonometry in a colony of glaucomatous dogs at 8, 15, 18, 20, and 30 months of age. Retinal function was evaluated using electroretinography (scotopic, photopic, and pattern). Examination of anterior segment structures was performed using gonioscopy and high-frequency ultrasonography (HFU). A gradual rise in IOP was observed with an increase in age: 8 months, 14 mm Hg (median value); 15 months, 15.5 mm Hg; 18 months, 17.5 mm Hg; 20 months, 24 mm Hg; 30 months, 36 mm Hg. Provocative testing with mydriatic agents (tropicamide and atropine 1%) caused significant increases in IOP (35% and 50%, respectively). HFU analysis showed complete collapse of iridocorneal angles by 20 months of age. Scotopic and photopic ERG analysis did not reveal significant deficits, but pattern ERG analysis showed significantly reduced amplitudes in glaucomatous dogs (glaucoma, 3.5 +/- 0.4 muV; control, 6.2 +/- 0.3 muV; P = 0.004; Student's t-test). Histologic analysis revealed collapse of the iridocorneal angle, posterior bowing of the lamina cribrosa, swelling and loss of large retinal ganglion cells, increased glial reactivity, and increased thickening of the lamina cribrosa. Canine hereditary angle-closure glaucoma is characterized by a progressive increase in intraocular pressure, loss of optic nerve function, and retinal ganglion cell loss.

Electroretinography

Eye Diseases, Hereditary - physiopathology

Optic Nerve Diseases - physiopathology

Intraocular Pressure

Dog Diseases - genetics

Anterior Eye Segment - pathology

Glial Fibrillary Acidic Protein - metabolism

Immunoenzyme Techniques

Optic Nerve Diseases - genetics

Eye Diseases, Hereditary - genetics

Eye Diseases, Hereditary - veterinary

Retinal Ganglion Cells - pathology

Glaucoma, Angle-Closure - physiopathology

Animals

Dog Diseases - physiopathology

Dogs

Optic Nerve Diseases - veterinary

Ultrasonography

Anterior Eye Segment - diagnostic imaging

Glaucoma, Angle-Closure - veterinary

Tonometry, Ocular

Gonioscopy

Disease Models, Animal

Glaucoma, Angle-Closure - genetics

Details

Title: Subtitle: Functional and structural changes in a canine model of hereditary primary angle-closure glaucoma
Creators: Sinisa D Grozdanic - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA. sgrozdan@iastate.edu
Helga Kecova
Matthew M Harper
Wijitha Nilaweera
Markus H Kuehn
Randy H Kardon
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.51(1), pp.255-263
DOI: 10.1167/iovs.09-4081
PMID: 19661222
PMCID: PMC3258664
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: R01 EY019485 / NEI NIH HHS
Language: English
Date published: 01/2010
Academic Unit: Iowa Neuroscience Institute; Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983979995502771

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