Journal article
Functional assays for replication protein A (RPA)
Methods in enzymology, Vol.409, pp.11-38
2006
DOI: 10.1016/S0076-6879(05)09002-6
PMID: 16793393
Abstract
Replication protein A (RPA) is a heterotrimeric, single-stranded DNA-binding protein. RPA is conserved in all eukaryotes and is essential for DNA replication, DNA repair, and recombination. RPA also plays a role in coordinating DNA metabolism and the cellular response to DNA damage. Assays have been established for many of these reactions. This chapter provides an overview of the methods used for analyzing RPA-DNA interactions, RPA-protein interactions, and functional activities of RPA. Methods are also discussed for visualizing RPA in the cell and analyzing the effects of RPA function on cell cycle progression in mammalian cells.
Details
- Title: Subtitle
- Functional assays for replication protein A (RPA)
- Creators
- Sara K Binz - Department of Biochemistry, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, USAAnne M DicksonStuart J HaringMarc S Wold
- Resource Type
- Journal article
- Publication Details
- Methods in enzymology, Vol.409, pp.11-38
- Publisher
- United States
- DOI
- 10.1016/S0076-6879(05)09002-6
- PMID
- 16793393
- ISSN
- 0076-6879
- eISSN
- 1557-7988
- Grant note
- R01 GM044721 / NIGMS NIH HHS
- Language
- English
- Date published
- 2006
- Academic Unit
- Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984024520302771
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