Functional characterization of retina and optic nerve after acute ocular ischemia in rats

Sinisa D Grozdanic; Donald S Sakaguchi; Young H Kwon; Randy H Kardon; Ioana M Sonea

doi:10.1167/iovs.02-0600

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Functional characterization of retina and optic nerve after acute ocular ischemia in rats

Journal article

Peer reviewed

Functional characterization of retina and optic nerve after acute ocular ischemia in rats

Sinisa D Grozdanic, Donald S Sakaguchi, Young H Kwon, Randy H Kardon and Ioana M Sonea

Investigative ophthalmology & visual science, Vol.44(6), pp.2597-2605

06/2003

DOI: 10.1167/iovs.02-0600

PMID: 12766062

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Abstract

To functionally characterize the status of the rat retina and optic nerve after acute elevation of intraocular pressure (IOP) and to determine the dynamics of the pathologic changes in the ischemic retina and optic nerve. Retinal ischemia was induced in rats by acutely increasing the IOP (110 mm Hg/60 minutes). Direct and indirect pupil light reflexes (PLRs) were recorded from the noninjured eye, and electroretinograms (flash and flicker ERG) were recorded from the injured and control eyes before and after surgery. Amplitudes and latencies were calculated for each recording session. Preoperative PLR(ratio)s (indirect/direct PLR) were 76.7 +/- 2.6 (mean +/- SEM). Twenty-four hours after surgery the PLR(ratio) was 15.2 +/- 12.8, 10 days after surgery, 11.6 +/- 9.8; 20 days after surgery, 26.5 +/- 8.0; and 28 days after surgery, 33.27 +/- 9.3. However, at day 35, the PLR had significantly recovered (41.1 +/- 7.3) when compared with the 24-hour postoperative ratios (P < 0.01, repeated-measures ANOVA). Forty-two days after surgery, the PLR(ratio) started to decrease once again in the injured eyes (28.7 +/- 5.9). Electroretinographic amplitudes (full-field flash ERG) followed a similar pattern. Cone responses (flicker ERG) were measured 42 days after surgery and revealed defects in injured eyes (control eyes: 46.6 +/- 2.9 microV, injured eyes: 3.4 +/- 1.7 microV). Histologic analysis revealed ischemic damage to all retinal layers, with the primary defects localized to the central retina. Acute ocular ischemia causes a significant decrease in retinal function, as measured by PLR and ERG, although over time the rat retina and optic nerve show partial regain of function.

Electroretinography

Optic Nerve - pathology

Acute Disease

Optic Nerve Diseases - physiopathology

Intraocular Pressure

Optic Nerve - blood supply

Rats

Ocular Hypertension - complications

Ischemia - physiopathology

Retinal Degeneration - physiopathology

Retinal Vessels - physiology

Optic Nerve Diseases - pathology

Reflex, Pupillary - physiology

Rats, Inbred BN

Animals

Reperfusion Injury - physiopathology

Photic Stimulation

Retinal Degeneration - pathology

Disease Models, Animal

Ischemia - etiology

Details

Title: Subtitle: Functional characterization of retina and optic nerve after acute ocular ischemia in rats
Creators: Sinisa D Grozdanic - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011, USA. sgrozdan@iastate.edu
Donald S Sakaguchi
Young H Kwon
Randy H Kardon
Ioana M Sonea
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.44(6), pp.2597-2605
DOI: 10.1167/iovs.02-0600
PMID: 12766062
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Language: English
Date published: 06/2003
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983980008402771

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