Journal article
Functional expression of frog and rainbow trout melanocortin 2 receptors using heterologous MRAP1s
General and comparative endocrinology, Vol.174(1), pp.5-14
2011
DOI: 10.1016/j.ygcen.2011.07.005
PMID: 21846469
Abstract
[Display omitted]
► Functional expression of
Xenopus tropicalis MC2R in CHO cells requires co-expression with mouse MRAP1. ► Functional expression of the
X. tropicalis MC2R is diminished when co-transfected with zebrafish MRAP1. ► Functional expression of
Oncorhynchus mykiss MC2R is robust when zebrafish MRAP is co-transfected into CHO cells. ► However, functional expression is marginal when the
O. mykiss MC2R is co-transfected with mouse MRAP1. ► It appears that there are teleost-specific and tetrapod-specific interactions between MC2R and MRAP1.
Analysis of the functional expression of the melanocortin 2 receptor (MC2R) from a rather broad spectrum of vertebrates indicates that MC2R is exclusively selective for the ligand, ACTH, and the melanocortin receptor accessory protein 1 (MRAP1) is required for high affinity ACTH binding and activation of MC2R. A phylogenetic analysis of MRAP1 suggested that tetrapod sequences and bony fish sequences may represent two distinct trends in the evolution of the
mrap1 gene. To test this hypothesis, a frog (
Xenopus tropicalis) MC2R was expressed in CHO cells either in the presence of a tetrapod (mouse) MRAP1 or a bony fish (zebrafish) MRAP1. The response of frog MC2R to different concentrations of human ACTH(1–24) was more robust in the presence of mouse MRAP1 than in the presence of zebrafish MRAP1. Conversely, the cAMP response mediated by the rainbow trout (
Oncorhynchus
mykiss) MC2R was almost twofold higher and occurred at 1000-fold lower ACTH concentration in the presence of zebrafish MRAP1 than in the presence of mouse MRAP1. Collectively, these experiments raise the possibility that at least two distinct trends have emerged in the co-evolution of MC2R/MRAP1 interactions during the radiation of the vertebrates.
Details
- Title: Subtitle
- Functional expression of frog and rainbow trout melanocortin 2 receptors using heterologous MRAP1s
- Creators
- Liang Liang - Department of Biological Sciences, University of Denver, Denver, CO 80210, United StatesJulien A Sebag - Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United StatesLauren Eagelston - Department of Biological Sciences, University of Denver, Denver, CO 80210, United StatesMadhavika N Serasinghe - Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United StatesKristopher Veo - Department of Biological Sciences, University of Denver, Denver, CO 80210, United StatesChristina Reinick - Department of Biological Sciences, University of Denver, Denver, CO 80210, United StatesJoseph Angleson - Department of Biological Sciences, University of Denver, Denver, CO 80210, United StatesPatricia M Hinkle - Department of Pharmacology and Physiology, University of Rochester Medical Center, Rochester, NY 14642, United StatesRobert M Dores - Department of Biological Sciences, University of Denver, Denver, CO 80210, United States
- Resource Type
- Journal article
- Publication Details
- General and comparative endocrinology, Vol.174(1), pp.5-14
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.ygcen.2011.07.005
- PMID
- 21846469
- ISSN
- 0016-6480
- eISSN
- 1095-6840
- Language
- English
- Date published
- 2011
- Academic Unit
- Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984070463902771
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