Journal article
Functional expression of the CXC-chemokine receptor-4/fusin on mouse microglial cells and astrocytes
The Journal of immunology (1950), Vol.159(2), pp.905-911
07/15/1997
DOI: 10.4049/jimmunol.159.2.905
PMID: 9218610
Abstract
The mRNA for the seven-transmembrane-spanning G protein-coupled receptor fusin/CXCR-4 is expressed in primary mouse astrocyte cultures and the transformed mouse microglial cell line, N9. Cell surface expression of fusin in these cells was confirmed by staining with a polyclonal anti-fusin Ab. The functional capacity of this chemokine receptor was examined by evaluating the calcium responses following stimulation of glial cells with the CXC-chemokine, stromal-derived cell factor-1alpha (SDF-1alpha). Both astrocytes and microglial cells mobilized calcium following stimulation with chemically synthesized SDF-1alpha. SDF-1alpha- and carbachol-mediated calcium responses of astrocytes were partially inhibited by treatment with pertussis toxin (PTx), suggesting receptor coupling to a combination of G alpha(i) and other G proteins. In contrast, the calcium responses of microglial cells to SDF-1alpha were completely PTx sensitive, while responses to carbachol stimulation were PTx resistant. The ability of SDF-1alpha to induce glial cell migration was also examined. Synthetic SDF-1alpha was a potent chemoattractant for mouse microglial cells at ligand concentrations of 10 to 500 ng/ml; peak responses were noted at 100 ng/ml. In contrast, astrocytes did not migrate toward a gradient of SDF-1alpha. The failure of SDF-1alpha to induce astrocyte migration was specific, as another chemokine, macrophage inflammatory protein-1alpha, triggered astrocyte chemotaxis.
Details
- Title: Subtitle
- Functional expression of the CXC-chemokine receptor-4/fusin on mouse microglial cells and astrocytes
- Creators
- Shigeyuki TanabeMichael Heesen - Harvard Medical SchoolIzumi YoshizawaMichael A BermanYi LuoConrad C BleulTimothy A SpringerKenji OkudaNorma GerardMartin E. Dorf
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.159(2), pp.905-911
- DOI
- 10.4049/jimmunol.159.2.905
- PMID
- 9218610
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 07/15/1997
- Academic Unit
- Urology
- Record Identifier
- 9984320851202771
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