Journal article
Functionalization of PCL-3D electrospun nanofibrous scaffolds for improved BMP2-induced bone formation
Applied materials today, Vol.10, pp.194-202
03/01/2018
DOI: 10.1016/j.apmt.2017.12.004
PMCID: PMC5863927
PMID: 29577064
Abstract
Bone morphogenic protein 2 (BMP2) is a key growth factor for bone regeneration, possessing FDA approvafor orthopedic applications. BMP2 is often required in supratherapeutic doses clinically, yielding adverse side effects and substantial treatment costs. Considering the crucial role of materials for BMPs delivery and cell osteogenic differentiation, we devote to engineering an innovative bone-matrix mimicking niche to improve low dose of BMP2-induced bone formation. Our previous work describes a novel technique, named thermally induced nanofiber self-agglomeration (TISA), for generating 3D electrospun nanofibrous (NF) polycaprolactone (PCL) scaffolds. TISA process could readily blend PCL with PLA, leading to increased osteogenic capabilities in vitro, however, these bio-inert synthetic polymers produced limited BMP2-induced bone formation in vivo. We therefore hypothesize that functionalization of NF 3DPCL scaffolds with bone-like hydroxyapatite ( HA) and BMP2 signaling activator phenamil will provide a favorable osteogenic niche for bone formation at low doses of BMP2. Compared to PCL-3D scaffolds, PCL/HA-3D scaffolds demonstrated synergistically enhanced osteogenic differentiation capabilities of C2C12 cells with phenamil. Importantly, in vivo studies showed that this synergism was able to generate significantly increased new bone in an ectopic mouse model, suggesting that PCL/HA-3D scaffolds act as a favorable synthetic extracellular matrix for bone regeneration. (c) 2017 Elsevier Ltd. All rights reserved.
Details
- Title: Subtitle
- Functionalization of PCL-3D electrospun nanofibrous scaffolds for improved BMP2-induced bone formation
- Creators
- Jacob M. Miszuk - University of South DakotaTao Xu - South Dakota School of Mines and TechnologyQingqing Yao - University of South DakotaFang Fang - Sanford ResearchJosh D. Childs - University of South DakotaZhongkui Hong - University of South DakotaPanning Tao - Sanford ResearchHao Fong - South Dakota School of Mines and TechnologyHongli Sun - University of South Dakota
- Resource Type
- Journal article
- Publication Details
- Applied materials today, Vol.10, pp.194-202
- DOI
- 10.1016/j.apmt.2017.12.004
- PMID
- 29577064
- PMCID
- PMC5863927
- NLM abbreviation
- Appl Mater Today
- ISSN
- 2352-9407
- eISSN
- 2352-9415
- Publisher
- Elsevier
- Number of pages
- 9
- Grant note
- P20 GM103620; P20 GM103548 / COBRE grants of National Institutes of Health UP1500172; UP1600205 / Competitive Research Grant program of South Dakota Board of Regents IIA-1335423 / EPSCoR program of National Science Foundation P20GM103620 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 1355423 / Office Of The Director; National Science Foundation (NSF); NSF - Office of the Director (OD)
- Language
- English
- Date published
- 03/01/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery; Dental Research
- Record Identifier
- 9984367737302771
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